Identification and structure of the nasR gene encoding a nitrate- and nitrite-responsive positive regulator of nasFEDCBA (nitrate assimilation) operon expression in Klebsiella pneumoniae M5al.

B S Goldman; J T Lin; V Stewart

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Identification and structure of the nasR gene encoding a nitrate- and nitrite-responsive positive regulator of nasFEDCBA (nitrate assimilation) operon expression in Klebsiella pneumoniae M5al.

机译：肺炎克雷伯菌M5al中nasFEDCBA（硝酸盐同化）操纵子表达的硝酸盐和亚硝酸盐响应性正调控子nasR基因的鉴定和结构。

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Klebsiella pneumoniae can use nitrate and nitrite as sole nitrogen sources through the nitrate assimilatory pathway. The structural genes for assimilatory nitrate and nitrite reductases together with genes necessary for nitrate transport form an operon, nasFEDCBA. Expression of the nasF operon is regulated both by general nitrogen control and also by nitrate or nitrite induction. We have identified a gene, nasR, that is necessary for nitrate and nitrite induction. The nasR gene, located immediately upstream of the nasFEDCBA operon, encodes a 44-kDa protein. The NasR protein shares carboxyl-terminal sequence similarity with the AmiR protein of Pseudomonas aeruginosa, the positive regulator of amiE (aliphatic amidase) gene expression. In addition, we present evidence that the nasF operon is not autogenously regulated.

机译：肺炎克雷伯菌可以通过硝酸盐吸收途径将硝酸盐和亚硝酸盐用作唯一的氮源。同化硝酸盐和亚硝酸盐还原酶的结构基因，以及硝酸盐运输所必需的基因，形成操纵子，nasFEDCBA。 nasF操纵子的表达既可以通过一般的氮控制，也可以通过硝酸盐或亚硝酸盐诱导来调节。我们已经确定了一个基因nasR，该基因对于诱导硝酸盐和亚硝酸盐是必需的。紧邻nasFEDCBA操纵子上游的nasR基因编码一个44 kDa的蛋白质。 NasR蛋白与铜绿假单胞菌的AmiR蛋白具有相同的羧基末端序列，铜绿假单胞菌是amiE（脂肪酰胺酶）基因表达的正向调节剂。此外，我们提供的证据表明nasF操纵子不受自身调节。

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《Journal of bacteriology》 |1994年第16期|共9页
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B S Goldman; J T Lin; V Stewart;
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1. Structures of genes nasA and nasB, encoding assimilatory nitrate and nitrite reductases in Klebsiella pneumoniae M5al. [J] . J T Lin, B S Goldman, V Stewart Journal of bacteriology . 1993,第8期

机译：肺炎克雷伯氏菌M5al中编码同化硝酸盐和亚硝酸盐还原酶的基因nasA和nasB的结构。
2. Genetic evidence that NarL function is not required for nitrate regulation of nitrate assimilation in Klebsiella pneumoniae M5al. [J] . V Stewart, B M Cali Journal of bacteriology . 1990,第8期

机译：遗传证据表明，肺炎克雷伯菌M5al中硝酸盐同化的硝酸盐调节不需要NarL功能。
3. Genetic regulation of nitrate assimilation in Klebsiella pneumoniae M5al. [J] . B M Cali, J L Micca, V Stewart Journal of bacteriology . 1989,第5期

机译：肺炎克雷伯菌M5al中硝酸盐同化的遗传调控。
4. Identification and structure of the nasR gene encoding a nitrate- and nitrite-responsive positive regulator of nasFEDCBA (nitrate assimilation) operon expression in Klebsiella pneumoniae M5al. [O] . B S Goldman, J T Lin, V Stewart 1994

机译：肺炎克雷伯菌M5al中nasFEDCBA（硝酸盐同化）操纵子表达的硝酸盐和亚硝酸盐响应性正调控子nasR基因的鉴定和结构。
5. Identification and structure of the nasR gene encoding a nitrate- and nitrite-responsive positive regulator of nasFEDCBA (nitrate assimilation) operon expression in Klebsiella pneumoniae M5al. [O] . Goldman, B S, Lin, J T, Stewart, V 1994

机译：肺炎克雷伯菌M5al中nasFEDCBA（硝酸盐同化）操纵子表达的硝酸盐和亚硝酸盐响应性正调控子nasR基因的鉴定和结构。

Identification and structure of the nasR gene encoding a nitrate- and nitrite-responsive positive regulator of nasFEDCBA (nitrate assimilation) operon expression in Klebsiella pneumoniae M5al.

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