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首页> 外文期刊>Journal of bacteriology >Expression of the Prevotella loescheii adhesin gene (plaA) is mediated by a programmed frameshifting hop.
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Expression of the Prevotella loescheii adhesin gene (plaA) is mediated by a programmed frameshifting hop.

机译:卢氏丙酸杆菌粘附素基因(plaA)的表达是由程序化的移码跃点介导的。

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摘要

The 2.4-kb plaA gene, which encodes a Prevotella loescheii galactoside-specific adhesin, contains a programmed frameshifting hop. The frameshift region consists of two UAA termination codons, two repeats of four identical bases between the terminators, and a stem-loop structure that has the potential to form a pseudoknot located downstream from the second UAA. The stem-loop and pseudoknot are features found in a number of retroviruses where frameshifting is a more common occurrence. The terminators, sequence repeats, and secondary structures were identified in both the P. loescheii plaA gene and the mRNA transcript. An in-frame fusion of the entire plaA frameshift region between codons 9 and 10 of the lacZ gene permitted relatively efficient expression (4 to 25% of that of the control) of beta-galactosidase in Escherichia coli.
机译:编码卢梭氏半乳糖半乳糖苷特异性粘附素的2.4 kb plaA基因包含一个已编程的移码跳。移码区域由两个UAA终止密码子,两个终止子之间的四个相同碱基组成的两个重复以及一个茎环结构构成,该结构可能形成位于第二个UAA下游的假结。茎环和假结是许多逆转录病毒中发现的特征,在这些逆转录病毒中,移码更为常见。在loescheii plaA基因和mRNA转录物中都鉴定出了终止子,序列重复序列和二级结构。 lacZ基因密码子9和10之间的完整plaA移码区的框内融合允许在大肠杆菌中相对有效地表达β-半乳糖苷酶(占对照的4%至25%)。

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