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首页> 外文期刊>Journal of bacteriology >Influence of different rol gene products on the chain length of Shigella dysenteriae type 1 lipopolysaccharide O antigen expressed by Shigella flexneri carrier strains.
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Influence of different rol gene products on the chain length of Shigella dysenteriae type 1 lipopolysaccharide O antigen expressed by Shigella flexneri carrier strains.

机译：不同的rol基因产物对痢疾志贺氏菌载体菌株表达的痢疾志贺氏菌1型脂多糖O抗原链长的影响。

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摘要

Introduction of the rol genes of Shigella dysenteriae 1 and Escherichia coli K-12 into Shigella flexneri carrier strains expressing the heterologous S. dysenteriae type 1 lipopolysaccharide resulted in the formation of longer chains of S. dysenteriae 1 O antigen. In bacteria producing both homologous and heterologous O antigen, this resulted in a reduction of the masking of heterologous O antigen by homologous lipopolysaccharide and an increased immune response induced by intraperitoneal immunization of mice by recombinant bacteria. The rol genes of S. dysenteriae 1 and E. coli K-12 were sequenced, and their gene products were compared with the S. flexneri Rol protein. The primary sequence of S. flexneri Rol differs from both E. coli K-12 and S. dysenteriae 1 Rol proteins only at positions 267 and 270, which suggests that this region may be responsible for the difference in biological activities.
机译：将痢疾志贺氏菌1和大肠杆菌K-12的rol基因导入表达异源痢疾链球菌1型脂多糖的弗氏志贺氏菌载体菌株中，导致痢疾链球菌1 O抗原的较长链形成。在同时产生同源和异源O抗原的细菌中，这导致同源脂多糖对异源O抗原的掩盖作用减少，并且重组细菌通过腹膜内免疫小鼠诱导了增强的免疫反应。对痢疾链球菌1和大肠杆菌K-12的rol基因进行测序，并将其基因产物与弗氏链球菌Rol蛋白进行比较。弗氏链球菌Rol的主要序列仅在267和270位与大肠杆菌K-12和痢疾链球菌1 Rol蛋白不同，这表明该区域可能是生物学活性差异的原因。

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