A Tn10 derivative (T-POP) for isolation of insertions with conditional (tetracycline-dependent) phenotypes.

C A Rappleye; J R Roth

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A Tn10 derivative (T-POP) for isolation of insertions with conditional (tetracycline-dependent) phenotypes.

机译：Tn10衍生物（T-POP），用于分离具有条件（四环素依赖性）表型的插入片段。

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A new Tn10-based transposon has been constructed and used to isolate insertion mutations with tetracycline-conditional phenotypes. Classes of mutants include conditional lethal mutations, conditional auxotrophs, and conditional mutants of the eut (ethanolamine utilization) operon. The described mutations were made with a new derivative of Tn10dTet that we have called Tn10d(T-POP). Others have noted that transposon Tn10dTet directs weak tetracycline-inducible transcripts out of both ends of the element into adjacent sequences. We have increased this level of outward transcription from Tn10dTet by selecting deletion mutations within the element that presumably remove transcription-termination signals. Insertion of the Tn10d(T-POP) element within an operon disrupts the target gene and makes expression of distal genes dependent on induction of outward transcription by tetracycline. Insertion mutations made with Tn10d(T-POP) can cause tetracycline-correctable conditional phenotypes based on expression of distal genes.

机译：一个新的基于Tn10的转座子已被构建并用于分离具有四环素条件表型的插入突变。突变体的种类包括条件致死突变，条件营养缺陷型和ut（操纵乙醇）操纵子的条件突变体。所描述的突变是由我们称为Tn10d（T-POP）的Tn10dTet新衍生物产生的。其他人已经注意到，转座子Tn10dTet将弱四环素诱导的转录本从元件的两端引出到相邻序列中。通过在推测可能去除转录终止信号的元件中选择缺失突变，我们提高了从Tn10dTet向外转录的水平。 Tn10d（T-POP）元件在操纵子中的插入会破坏目标基因，并使远端基因的表达依赖于四环素的向外转录诱导。 Tn10d（T-POP）引起的插入突变可导致基于远端基因表达的可校正四环素的条件表型。

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《Journal of bacteriology》 |1997年第18期|共8页
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C A Rappleye; J R Roth;
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1. A Tn10 derivative (T-POP) for isolation of insertions with conditional (tetracycline-dependent) phenotypes. [J] . C A Rappleye, J R Roth Journal of bacteriology . 1997,第18期

机译：Tn10衍生物（T-POP），用于分离具有条件（四环素依赖性）表型的插入片段。
2. P-lacW insertional mutagenesis on the second chromosome of Drosophila melanogaster: isolation of lethals with different overgrowth phenotypes. [J] . G Tick, I Kiss, M Alvarado, Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation . 1993,第1期

机译：在果蝇的第二条染色体上进行P-lacW插入诱变：分离具有不同过度生长表型的致死菌。
3. P-lacW insertional mutagenesis on the second chromosome of Drosophila melanogaster: isolation of lethals with different overgrowth phenotypes. [J] . G Tick, I Kiss, M Alvarado, Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation . 1993,第1期

机译：在果蝇的第二条染色体上进行P-lacW插入诱变：分离具有不同过度生长表型的致死菌。
4. Immediate Conditional Insertion Sort (ICIS) [C] . Romani Farid Ibrahim IEEE South East Conference . 2020

机译：立即条件插入排序（ICIS）
5. Part I. Chemoenzymatic synthesis of usnic acid and analogues of usnic acid. Part II. The isolation of citric acid derivatives from Aspergillus niger. [D] . Hawranik, Drew Jonathon. 2010

机译：第一部分：松萝酸和松萝酸类似物的化学酶法合成。第二部分从黑曲霉中分离柠檬酸衍生物。
6. A Tn10 derivative (T-POP) for isolation of insertions with conditional (tetracycline-dependent) phenotypes. [O] . C A Rappleye, J R Roth 1997

机译：Tn10衍生物（T-POP）用于分离具有条件（四环素依赖性）表型的插入片段。
7. A Tn10 derivative (T-POP) for isolation of insertions with conditional (tetracycline-dependent) phenotypes. [O] . Rappleye, C A, Roth, J R 1997

机译：Tn10衍生物（T-POP），用于分离具有条件（四环素依赖性）表型的插入片段。

A Tn10 derivative (T-POP) for isolation of insertions with conditional (tetracycline-dependent) phenotypes.

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