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首页> 外文期刊>Journal of bacteriology >Osmoregulated Periplasmic Glucans ofErwinia chrysanthemi
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Osmoregulated Periplasmic Glucans ofErwinia chrysanthemi

机译：菊花欧文氏菌的渗透性周质葡聚糖

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We report the initial characterization of the osmoregulated periplasmic glucans (OPGs) of Erwinia chrysanthemi. OPGs are intrinsic components of the bacterial envelope necessary to the pathogenicity of this phytopathogenic enterobacterium (F. Page, S. Altabe, N. Hugouvieux-Cotte-Pattat, J.-M. Lacroix, J. Robert-Baudouy and J.-P. Bohin, J. Bacteriol. 183:0000–0000, 2001 [companion in this issue]). OPGs were isolated by trichloracetic acid treatment and gel permeation chromatography. The synthesis of these compounds appeared to be osmoregulated, since lower amounts of OPGs were produced when bacteria were grown in media of higher osmolarities. However, a large fraction of these OPGs were recovered in the culture medium. Then, these compounds were characterized by compositional analysis, high-performance anion-exchange chromatography, matrix-assisted laser desorption mass spectrometry, and 1H and 13C nuclear magnetic resonance analyses. OPGs produced by E. chrysanthemi are very heterogeneous at the level of both backbone structure and substitution of these structures. The degree of polymerization of the glucose units ranges from 5 to 12. The structures are branched, with a linear backbone consisting of β-1,2-linked glucose units to which a variable number of branches, composed of one glucose residue, are attached by β-1,6 linkages in a random way. This glucan backbone may be substituted by O-acetyl andO-succinyl ester-linked residues.
机译：我们报告渗透性周质葡聚糖（OPGs）的欧文氏菊的初步表征。 OPG是这种植物致病性肠杆菌致病性所必需的细菌包膜的固有成分（F. Page，S. Altabe，N.Hugouvieux-Cotte-Pattat，J.-M. Lacroix，J.Robert-Baudouy和J.-P Bohin，J. Bacteriol。183：0000–0000，2001年[此期的对应内容]）。通过三氯乙酸处理和凝胶渗透色谱分离OPG。这些化合物的合成似乎被渗透调节，因为当细菌在较高渗透压的培养基中生长时，产生的OPG量较少。然而，这些OPG的很大一部分是在培养基中回收的。然后，通过组成分析，高效阴离子交换色谱，基质辅助激光解吸质谱， 1 H和 13 C核磁共振分析对这些化合物进行表征。 。由 E生产的OPG。 chrysanthemi 在骨架结构和这些结构的取代方面都非常异质。葡萄糖单元的聚合度为5至12。该结构是分支的，其线性骨架由β-1,2-连接的葡萄糖单元组成，其上连接有由一个葡萄糖残基组成的可变数目的分支。通过β-1,6连锁以随机方式该葡聚糖主链可以被 O -乙酰基和 O -琥珀酰酯连接的残基取代。

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