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首页> 外文期刊>Journal of bacteriology >Osmoregulated Periplasmic Glucan Synthesis Is Required for Erwinia chrysanthemi Pathogenicity
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Osmoregulated Periplasmic Glucan Synthesis Is Required for Erwinia chrysanthemi Pathogenicity

机译:渗透压的周质葡聚糖合成是必需的菊花欧文氏菌致病性。

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Erwinia chrysanthemi is a phytopathogenic enterobacterium causing soft rot disease in a wide range of plants. Osmoregulated periplasmic glucans (OPGs) are intrinsic components of the gram-negative bacterial envelope. We cloned the opgGHoperon of E. chrysanthemi, encoding proteins involved in the glucose backbone synthesis of OPGs, by complementation of the homologous locus mdoGH of Escherichia coli. OpgG and OpgH show a high level of similarity with MdoG and MdoH, respectively, and mutations in the opgG or opgHgene abolish OPG synthesis. The opg mutants exhibit a pleiotropic phenotype, including overproduction of exopolysaccharides, reduced motility, bile salt hypersensitivity, reduced protease, cellulase, and pectate lyase production, and complete loss of virulence. Coinoculation experiments support the conclusion that OPGs present in the periplasmic space of the bacteria are necessary for growth in the plant host.
机译:欧文氏菌(Erwinia chrysanthemi)是一种植物致病性肠杆菌,可在多种植物中引起软腐病。渗透压的周质葡聚糖(OPG)是革兰氏阴性细菌包膜的固有成分。我们克隆了 E的 opgGH 操纵子。 chrysanthemi ,通过与大肠杆菌的 mdoGH 同源位点互补,编码参与OPGs葡萄糖骨架合成的蛋白质。 OpgG和OpgH分别显示与MdoG和MdoH的高度相似,并且 opgG opgH 基因中的突变废除了OPG合成。 opg 突变体表现出多效性表型,包括胞外多糖过量产生,运动性降低,胆汁盐超敏性,蛋白酶,纤维素酶和果胶酸裂合酶产量降低,以及完全丧失毒力。共接种实验支持以下结论:存在于细菌周质空间中的OPG对于植物宿主的生长是必需的。

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