Structure-Function Analysis of BfpB, a Secretin-Like Protein Encoded by the Bundle-Forming-Pilus Operon of EnteropathogenicEscherichia coli

Sarah A. Schmidt; David Bieber; Sandra W. Ramer; Jaiweon Hwang; Cheng-Yen Wu; Gary Schoolnik

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Structure-Function Analysis of BfpB, a Secretin-Like Protein Encoded by the Bundle-Forming-Pilus Operon of EnteropathogenicEscherichia coli

机译：BfpB的结构功能分析，这种蛋白由肠致病性大肠杆菌的束形成菌毛操纵子编码。

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Production of type IV bundle-forming pili by enteropathogenicEscherichia coli (EPEC) requires BfpB, an outer-membrane lipoprotein and member of the secretin protein superfamily. BfpB was found to compose a ring-shaped, high-molecular-weight outer-membrane complex that is stable in 4% sodium dodecyl sulfate at temperatures of ≤65°C. Chemical cross-linking and immunoprecipitation experiments disclosed that the BfpB multimeric complex interacts with BfpG, and mutational studies showed that BfpG is required for the formation and/or stability of the multimer but not for the outer-membrane localization of BfpB. Formation of the BfpB multimer also does not require BfpA, the repeating subunit of the pilus filament. Functional studies of the BfpB-BfpG complex revealed that its presence confers vancomycin sensitivity, indicating that it may form an incompletely gated channel through the outer membrane. BfpB expression is also associated with accumulation of EPEC proteins in growth medium, suggesting that it may support both pilus biogenesis and protein secretion.

机译：肠致病性大肠埃希氏菌（EPEC）产生IV型成束菌毛需要BfpB，外膜脂蛋白和促胰液素蛋白超家族成员。发现BfpB组成了一个环状的高分子量外膜复合物，该复合物在≤65°C的温度下在4％的十二烷基硫酸钠中稳定。化学交联和免疫沉淀实验表明BfpB多聚体复合物与BfpG相互作用，突变研究表明BfpG是多聚体形成和/或稳定性所必需的，而不是BfpB的外膜定位所必需的。 BfpB多聚体的形成也不需要BfpA（菌毛丝的重复亚基）。 BfpB-BfpG复合物的功能研究表明，它的存在赋予万古霉素敏感性，表明它可能形成穿过外膜的不完全封闭的通道。 BfpB的表达还与EPEC蛋白在生长培养基中的积累有关，表明它可能支持菌毛的生物发生和蛋白的分泌。

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《Journal of bacteriology》 |2001年第16期|共12页
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Sarah A. Schmidt; David Bieber; Sandra W. Ramer; Jaiweon Hwang; Cheng-Yen Wu; Gary Schoolnik;
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1. Characterization of the tol-pal and cyd region of Escherichia coli K-12: transcript analysis and identification of two new proteins encoded by the cyd operon. [J] . M M Muller, R E Webster Journal of bacteriology . 1997,第6期

机译：大肠杆菌K-12的tol-pal和cyd区域的特征：转录本分析和cyd操纵子编码的两种新蛋白质的鉴定。
2. Characterization of the tol-pal and cyd region of Escherichia coli K-12: transcript analysis and identification of two new proteins encoded by the cyd operon. [J] . M M Muller, R E Webster Journal of bacteriology . 1997,第6期

机译：大肠杆菌K-12的tol-pal和cyd区域的特征：转录本分析和cyd操纵子编码的两种新蛋白质的鉴定。
3. Transcriptional Regulation of the orf19 Gene and thetir-cesT-eae Operon of EnteropathogenicEscherichia coli [J] . Claudia Sánchez-SanMart??n, V??ctor H. Bustamante, Edmundo Calva, Journal of bacteriology . 2001,第9期

机译：肠道致病性大肠杆菌的orf19基因和tess-ceT-eae操纵子的转录调控
4. Operon prediction based on quadratic discriminant analysis in Escherichia coli genome [C] . Meizhen Ji, Lu Jun, Zhang Ying, The 2nd International Conference on Information Science and Engineering . 2010

机译：基于二次判别分析的大肠杆菌基因组预测操纵子
5. Escherichia coli DNA helicase II: Structure-function analysis of conserved amino acid motifs and investigation of protein-protein interactions [D] . Hall, Mark Christopher. 1998

机译：大肠杆菌DNA解旋酶II：保守氨基酸基序的结构功能分析和蛋白质-蛋白质相互作用的研究
6. Structure-Function Analysis of BfpB a Secretin-Like Protein Encoded by the Bundle-Forming-Pilus Operon of Enteropathogenic Escherichia coli [O] . Sarah A. Schmidt, David Bieber, Sandra W. Ramer, 2001

机译：BfpB的结构功能分析这种蛋白由肠致病性大肠杆菌的束形成菌毛操纵子编码。
7. Structure-Function Analysis of BfpB, a Secretin-Like Protein Encoded by the Bundle-Forming-Pilus Operon of Enteropathogenic Escherichia coli [O] . Schmidt, Sarah A., Bieber, David, Ramer, Sandra W., 2001

机译：BfpB的结构功能分析，这种蛋白由肠致病性大肠杆菌的束形成菌毛操纵子编码。

Structure-Function Analysis of BfpB, a Secretin-Like Protein Encoded by the Bundle-Forming-Pilus Operon of EnteropathogenicEscherichia coli

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