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首页> 外文期刊>Journal of bacteriology >Effects of salt and temperature on plasmid topology in the halophilic archaeon Haloferax volcanii.
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Effects of salt and temperature on plasmid topology in the halophilic archaeon Haloferax volcanii.

机译：盐和温度对嗜盐古细菌Haloferax volcanii中质粒拓扑的影响。

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摘要

We report here the effect of environmental parameters, salinity, temperature, and an intercalating drug on plasmid topology in the halophilic archaeon Haloferax volcanii. We first studied the topological state of the plasmid pHV11 in media of different salt compositions and concentrations. The superhelical density of plasmid PHV11 varies in a way that depends on the kind of salt and on the concentrations of individual salts. With respect to growth temperature, the plasmid linking number increased at higher temperature in a linear way, contrary to what has been reported for Escherichia coli, in which the plasmid linking number decreased at higher temperature. These results suggest that some of the mechanisms that control DNA supercoiling in halophilic Archaea may be different from those described for E. coli. However, homeostatic control of DNA supercoiling seems to occur in haloarchaea, as in Bacteria, since we found that relaxation of DNA by chloroquine triggers an increase in negative supercoiling.
机译：我们在这里报告了环境参数，盐度，温度和嵌入药物对嗜盐古细菌Haloferax volcanii中质粒拓扑结构的影响。我们首先研究了质粒pHV11在不同盐组成和浓度的培养基中的拓扑状态。质粒PHV11的超螺旋密度的变化取决于盐的种类和各个盐的浓度。关于生长温度，质粒连接数在较高温度下以线性方式增加，这与大肠杆菌的报道相反，在大肠杆菌中，质粒连接数在较高温度下降低。这些结果表明，在嗜盐古生菌中控制DNA超螺旋的某些机制可能与针对大肠杆菌所描述的机制不同。但是，由于细菌发现氯喹使DNA松弛引起负超螺旋的增加，因此与细菌一样，似乎在嗜盐菌中也发生了对DNA超螺旋的稳态控制。

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