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首页> 外文期刊>Journal of bacteriology >Vibrio cholerae O139 Bengal: Combined Physical and Genetic Map and Comparative Analysis with the Genome of V. cholerae O1
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Vibrio cholerae O139 Bengal: Combined Physical and Genetic Map and Comparative Analysis with the Genome of V. cholerae O1

机译：霍乱弧菌O139孟加拉：结合的物理和遗传图谱以及与霍乱弧菌O1基因组的比较分析

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摘要

A combined physical and genetic map of the genome of strain SG24 ofVibrio cholerae O139 Bengal, a novel non-O1 strain having epidemic potential, has been constructed by using the enzymesNotI, SfiI, and CeuI. The genome of SG24 is circular, and the genome size is about 3.57 Mb. The linkages between 47 NotI and 32 SfiI fragments ofV. cholerae SG24 genomic DNA were determined by combining two approaches: (i) identification of fragments produced by enzyme I in fragments produced by enzyme II by the method of fragment excision, redigestion, and end labeling and (ii) use of the linking clone libraries generated from the genome of classical O1 strain 569B. The linkages between nine CeuI fragments were determined primarily by analyses of partial fragments of theCeuI-digested genome. More than 80 cloned homologous and heterologous genes, including several operons, have been positioned on the physical map. The map of the SG24 genome represents the second map of a V. cholerae genome, and a comparison of this map with that of classical O1 strain 569B revealed considerable diversity in DNA restriction sites and allowed identification of hypervariable regions. Several genetic markers, including virulence determinant genes, are in different positions in the SG24 and 569B genomes.
机译：利用酶 Not 构建了霍乱弧菌 O139孟加拉SG24菌株基因组的物理和遗传图谱，该菌株是一种具有流行潜力的新型非O1菌株。 I， Sfi I和 Ceu I。 SG24的基因组是环状的，基因组大小约为3.57 Mb。 47个 Not I和32个 V的 Sfi I片段之间的联系。霍乱弧菌SG24基因组DNA的检测是通过结合两种方法来进行的：（i）通过片段切除，重排和末端标记的方法鉴定酶II产生的片段中酶I产生的片段，以及（ii）使用连接从经典O1菌株569B的基因组生成的克隆文库。九个 Ceu I片段之间的联系主要是通过分析 Ceu I消化的基因组的部分片段来确定的。在物理图谱上已定位了80多个克隆的同源和异源基因，包括几个操纵子。 SG24基因组的图谱代表 V的第二个图谱。该图谱与经典O1菌株569B的图谱比较表明，DNA限制性酶切位点具有很大的多样性，并可以鉴定高变区。 SG24和569B基因组中的几种遗传标记（包括毒力决定基因）位于不同的位置。

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