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METHOD FOR MOLECULAR GENETIC TYPING OF V. cholerae O1 AND O139 SEROGROUP

机译:霍乱弧菌O1和O139血清群的分子遗传分型方法

摘要

The method of molecular genetic typing of O1 and O139 serogroups, including the isolation of DNA from the studied culture, PCR staging with specific primers and taking into account the reaction by electrophoresis, characterized in that the amplification of the studied DNA is carried out with specific specific primers for each of the six INDEL genes, namely: ompU with primers AACAAGACTCTGATTGCTCTTGC STTTSAGAGATAGGCGAGCTTC, 1606 with primers ATGCCACACAAAACCTCAATCTA AATATCGCGTTCAGTGATGGTAG, eamA with primers TCAGCTTATCTCAGTGGTTATGGA AGAGGCAGAATAATCATTCCAATC, 096 with primers CATGAATTAGGGCATAACTTTGG GCCCTTTAATCCCATAACCATAA, CoA with primers CATCATTTACAGCATTGCCAGT CTTCACC AATCGGCTTAATCG, cheA with ACTTCGCGAGATTCATCAGAA CTGTTTCTAGTGATGCGAAAGC primers, each of the identified INDEL genes has two discriminated alleles, typing results are taken into account as visual identification after electrophoresis, determining a unique “top” or “bottom” strain for the studied EL and IND -genotype by two or more INDEL genes and by comparing the latter with a reference marker determine the belonging to the O1 and O139 serogroups.
机译:O1和O139血清群的分子遗传分型方法,包括从研究的培养物中分离DNA,使用特异性引物进行PCR分期以及考虑到电泳反应,其特征在于,研究的DNA扩增可通过特异性ompU用引AACAAGACTCTGATTGCTCTTGC STTTSAGAGATAGGCGAGCTTC,1606引物ATGCCACACAAAACCTCAATCTA AATATCGCGTTCAGTGATGGTAG,EAMA用引TCAGCTTATCTCAGTGGTTATGGA AGAGGCAGAATAATCATTCCAATC,096用引CATGAATTAGGGCATAACTTTGG GCCCTTTAATCCCATAACCATAA,辅酶A用引CATCATTTACAGCATTGCCAGT CTTCACC AATCGGCTTAATCG,的cheA与ACTTCGCGAGATTCATCAGAA CTGTTTCTAGTGATGCGAAAGC引物,每个:对于6个INDEL基因,即特异性引物个已鉴定的INDEL基因具有两个可区分的等位基因,电泳后的目视鉴定应考虑打字结果,确定所研究的EL和IND基因型的唯一“顶部”或“底部”菌株为两个或两个更多INDEL基因,并通过将后者与参考标记进行比较,确定属于O1和O139血清群。

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