Bacillus subtilis Cell Cycle as Studied by Fluorescence Microscopy: Constancy of Cell Length at Initiation of DNA Replication and Evidence for Active Nucleoid Partitioning

Michaela E. Sharpe; Philippe M. Hauser; Robert G. Sharpe; Jeffery Errington

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Bacillus subtilis Cell Cycle as Studied by Fluorescence Microscopy: Constancy of Cell Length at Initiation of DNA Replication and Evidence for Active Nucleoid Partitioning

机译：通过荧光显微镜研究枯草芽孢杆菌的细胞周期：DNA复制开始时细胞长度的恒定性和主动核样分区的证据

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Fluorescence microscopic methods have been used to characterize the cell cycle of Bacillus subtilis at four different growth rates. The data obtained have been used to derive models for cell cycle progression. Like that of Escherichia coli, the period required by B. subtilis for chromosome replication at 37°C was found to be fairly constant (although a little longer, at about 55 min), as was the cell mass at initiation of DNA replication. The cell cycle of B. subtilis differed from that ofE. coli in that changes in growth rate affected the average cell length but not the width and also in the relative variability of period between termination of DNA replication and septation. Overall movement of the nucleoid was found to occur smoothly, as in E. coli, but other aspects of nucleoid behavior were consistent with an underlying active partitioning machinery. The models for cell cycle progression in B. subtilis should facilitate the interpretation of data obtained from the recently introduced cytological methods for imaging the assembly and movement of proteins involved in cell cycle dynamics.

机译：荧光显微镜方法已被用来表征四种不同生长速率下枯草芽孢杆菌的细胞周期。获得的数据已用于推导细胞周期进程的模型。与大肠杆菌一样，也是 B所要求的时间。发现在37°C下用于染色体复制的枯草芽孢杆菌相当稳定（尽管更长，大约在55分钟），DNA复制开始时的细胞团也是如此。 B的细胞周期。枯草与 E不同。大肠杆菌的变化在于生长速度的变化影响平均细胞长度，而不影响宽度，并且影响DNA复制终止和分离之间的时间间隔的相对变异性。如在 E中那样，发现核苷的整体运动平稳进行。大肠杆菌，但是其他类核苷酸行为与潜在的主动分配机制是一致的。 B中细胞周期进程模型。枯草杆菌应该有助于解释从最近引入的细胞学方法获得的数据，这些方法用于对参与细胞周期动力学的蛋白质的组装和运动进行成像。 展开▼

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《Journal of bacteriology》 |1998年第3期|共9页

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Michaela E. Sharpe; Philippe M. Hauser; Robert G. Sharpe; Jeffery Errington;
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专利

1. Subcellular localization of Dna-initiation proteins of Bacillus subtilis: evidence that chromosome replication begins at either edge of the nucleoids. [J] . Imai Y, Ogasawara N, Ishigo Oka-D, Molecular Microbiology . 2000,第5期

机译：枯草芽孢杆菌Dna起始蛋白的亚细胞定位：证据表明染色体复制始于核苷酸的任一边缘。

2. Subcellular positioning of the origin region of the Bacillus subtilis chromosome is independent of sequences within oriC, the site of replication initiation, and the replication initiator DnaA [J] . Berkmen MB, Grossman AD Molecular Microbiology . 2007,第1期

机译：枯草芽孢杆菌染色体起源区域的亚细胞定位与oriC中的序列，复制起始位点和复制起始剂DnaA无关

3. DNA gyrase activity regulates DnaA‐dependent replication initiation in Bacillus subtilis Bacillus subtilis [J] . Samadpour A. N., Merrikh H. Molecular Microbiology . 2018,第2期

机译：DNA丙氨酸活性调节枯草芽孢杆菌枯草芽孢杆菌的DNAA依赖性复制起始

4. EXCITATION ENERGY TRANSFER FROM PHYCOBILIPROTEIN TO CHLOROPHYLL d IN INTACT CELLS OF ACARYOCHLORIS MARINA STUDIED BY TIME- AND WAVELENGTH-RESOLVED FLUORESCENCE SPECTROSCOPY [C] . Hann-Jorg Eckert, Zdenek Petrasek, Christoph Theiss, International Congress of Photosynthesis . 2005

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5. Re-Initiation Promoters: Genetic Elements that Modify Cell Cycle Control of Adjacent DNA Replication Origins [D] . Richardson, Christopher Douglas 2014

机译：重新启动子：遗传因素，改变相邻的DNA复制起点的细胞周期控制。

6. Bacillus subtilis Cell Cycle as Studied by Fluorescence Microscopy: Constancy of Cell Length at Initiation of DNA Replication and Evidence for Active Nucleoid Partitioning [O] . Michaela E. Sharpe, Philippe M. Hauser, Robert G. Sharpe, 1998

机译：枯草芽孢杆菌细胞周期的荧光显微镜研究：DNA复制开始时细胞长度的恒定性和主动核样分区的证据

7. Bacillus subtilis Cell Cycle as Studied by Fluorescence Microscopy: Constancy of Cell Length at Initiation of DNA Replication and Evidence for Active Nucleoid Partitioning [O] . Michaela E. Sharpe, Philippe M. Hauser, Robert G. Sharpe, 1998

机译：由荧光显微镜研究的芽孢杆菌细胞周期：在DNA复制开始时细胞长度的恒定和活性核序列的证据

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3. 真核细胞染色体DNA复制起始及复制叉稳定性维持的机制 [J] . 吴丽虹 ,刘阳 ,孔道春 . 中国科学 . 2013,第010期

4. 黄芩素对喉癌细胞周期、凋亡率及细胞核DNA的影响 [J] . 孙吉凤 ,刘剑凯 . 中国医学创新 . 2018,第016期

5. 周期蛋白6在DNA复制、细胞周期检测点和肿瘤发生发展中的作用 [J] . 张佳华 ,黄士昂 . 国际肿瘤学杂志 . 2009,第006期

6. 乙型肝炎病毒复制患者外周血单个核细胞中HBV DNA和cccDNA的定量检测 [C] . 钟艳丹 ,谭德明 ,杨永峰 . 第12次长江三角洲公共卫生临床体系建设学术研讨会 . 2007

7. DOC-1R蛋白抑制细胞周期S期复制和CDK2序列影响其入核的初步研究 [A] . 刘琦 . 2004

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Bacillus subtilis Cell Cycle as Studied by Fluorescence Microscopy: Constancy of Cell Length at Initiation of DNA Replication and Evidence for Active Nucleoid Partitioning

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