Mutational Analysis of the Phosphate-Binding Loop of Rhizobium meliloti DctD, a ?54-Dependent Activator

Yan Gao; Ying-Kai Wang; Timothy R. Hoover

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Mutational Analysis of the Phosphate-Binding Loop of Rhizobium meliloti DctD, a ?54-Dependent Activator

机译：突变根瘤菌DctD，一个？54依赖激活剂的磷酸盐结合环的突变分析。

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The phosphate-binding loop of ?54-dependent activators is thought to participate in ATP binding and/or hydrolysis. Alanine substitutions at positions 3, 4, 6, 7, and 8 of this motif inRhizobium meliloti DctD disrupted transcriptional activation and ATP hydrolysis. Interestingly, substitution of alanine at position 7 also affected DNA binding.

机译：认为α 54 依赖性活化剂的磷酸结合环参与ATP结合和/或水解。苜蓿根瘤菌DctD中该基序的3、4、6、7和8位上的丙氨酸取代破坏了转录激活和ATP水解。有趣的是，第7位的丙氨酸取代也影响DNA结合。

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《Journal of bacteriology》 |1998年第10期|共4页
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Yan Gao; Ying-Kai Wang; Timothy R. Hoover;
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1. Rhizobium meliloti and Rhizobium leguminosarum dctD gene products bind to tandem sites in an activation sequence located upstream of sigma 54-dependent dctA promoters. [J] . H Ledebur, B Gu, J Sojda, Journal of bacteriology . 1990,第7期

机译：苜蓿根瘤菌和豆科根瘤菌dctD基因产物与位于sigma 54依赖性dctA启动子上游的激活序列中的串联位点结合。
2. Mutational Analysis of the Rhizobium lupini H13-3 andSinorhizobium meliloti Flagellin Genes: Importance of Flagellin A for Flagellar Filament Structure and Transcriptional Regulation [J] . Birgit Scharf, Henriette Schuster-Wolff-Bühring, Reinhard Rachel, Journal of bacteriology . 2001,第18期

机译：突变根瘤菌H13-3和Sinorhizobium meliloti鞭毛蛋白基因的突变分析：鞭毛蛋白A对鞭毛丝结构和转录调控的重要性。
3. Genetic Analysis of Mutations Affecting pckA Regulation in Rhizobium (Sinorhizobium) meliloti [J] . Magne ?ster?s, Shelley A. P. OBrien, Turlough M. Finan Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation . 1997,第4期

机译：苜蓿根瘤菌中影响pckA调控的突变体的遗传分析
4. Phosphoenolpyruvate carboxylase (PEPC): mutational analysis of a flexible loop and a putative binding site for an allosteric activator, glucose 6-phosphate (G6P) [C] . A Terada, M Kotera, K Tsumura, International Congress on Photosynthesis . 2001

机译：磷酸丙烯酸盐羧基（Pepc）：柔性环的突变分析和血糖活化剂的推定结合位点，葡萄糖6-磷酸（G6P）
5. Identification and characterization of upstream elements required for the transcription of the C(4)-dicarboxylate transport gene, dctA, in Rhizobium meliloti and Rhizobium leguminosarum [D] . Ledebur, Harry Calhoon, Jr. 1992

机译：鉴定和表征C（4）-二羧酸盐运输基因dctA转录的根瘤菌和根瘤菌中所需的上游元素
6. Note: Mutational Analysis of the Phosphate-Binding Loop of Rhizobium meliloti DctD a ς54-Dependent Activator [O] . Yan Gao, Ying-Kai Wang, Timothy R. Hoover 1998

机译：注意：根瘤菌54依赖性激活剂根瘤菌DctD的磷酸盐结合环的突变分析
7. Rhizobium meliloti and Rhizobium leguminosarum dctD gene products bind to tandem sites in an activation sequence located upstream of sigma 54-dependent dctA promoters. [O] . Ledebur, H, Gu, B, Sojda, J, 1990

机译：苜蓿根瘤菌和豆科根瘤菌dctD基因产物与位于sigma 54依赖性dctA启动子上游的激活序列中的串联位点结合。

Mutational Analysis of the Phosphate-Binding Loop of Rhizobium meliloti DctD, a ?54-Dependent Activator

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