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首页> 外文期刊>Journal of bacteriology >Transduction by φBB-1, a Bacteriophage ofBorrelia burgdorferi
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Transduction by φBB-1, a Bacteriophage ofBorrelia burgdorferi

机译：伯氏疏螺旋体噬菌体φBB-1的转导

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We previously described a bacteriophage of the Lyme disease agentBorrelia burgdorferi designated φBB-1. This phage packages the host complement of the 32-kb circular plasmids (cp32s), a group of homologous molecules found throughout the genusBorrelia. To demonstrate the ability of φBB-1 to package and transduce DNA, a kanamycin resistance cassette was inserted into a cloned fragment of phage DNA, and the resulting construct was transformed into B. burgdorferi CA-11.2A cells. The kan cassette recombined into a resident cp32 and was stably maintained. The cp32 containing the kan cassette was packaged by φBB-1 released from this B. burgdorferi strain. φBB-1 has been used to transduce this antibiotic resistance marker into naive CA-11.2A cells, as well as two other strains of B. burgdorferi. This is the first direct evidence of a mechanism for lateral gene transfer in B. burgdorferi.
机译：我们之前曾描述过一种名为BBBB-1的莱姆病病原菌 Borrelia burgdorferi 的噬菌体。该噬菌体包装了32kb环状质粒（cp32s）的宿主互补序列，cp32s是在整个番茄属中发现的一组同源分子。为了证明φBB-1包装和转导DNA的能力，将卡那霉素抗性盒插入克隆的噬菌体DNA片段中，并将所得构建体转化为 B。 burgdorferi CA-11.2A细胞。 kan 盒带重组为驻留cp32，并得以稳定维护。包含 kan 盒带的cp32由从该 B释放的φBB-1包装。 burgdorferi 菌株。 φBB-1已被用于将该抗生素抗性标记物转导至幼稚的CA-11.2A细胞以及另外两个 B菌株。 burgdorferi 。这是 B中横向基因转移机制的第一个直接证据。 burgdorferi 。

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