• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Multiple Promoter Inversions Generate Surface Antigenic Variation in Mycoplasma penetrans
【24h】

Multiple Promoter Inversions Generate Surface Antigenic Variation in Mycoplasma penetrans

机译：多个启动子反转在支原体支原体中产生表面抗原变异

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mycoplasma penetrans is a newly identified species of the genus Mycoplasma. It was first isolated from a urine sample from a human immunodeficiency virus (HIV)-infected patient. M. penetrans changes its surface antigen profile with high frequency. The changes originate from ON?OFF phase variations of the P35 family of surface membrane lipoproteins. The P35 family lipoproteins are major antigens recognized by the human immune system during M. penetrans infection and are encoded by the mpl genes. Phase variations of P35 family lipoproteins occur at the transcriptional level of mpl genes; however, the precise genetic mechanisms are unknown. In this study, the molecular mechanisms of surface antigen profile change in M. penetrans were investigated. The focus was on the 46-kDa protein that is present in M. penetrans strain HF-2 but not in the type strain, GTU. The 46-kDa protein was the product of a previously reported mpl gene, pepIMP13, with an amino-terminal sequence identical to that of the P35 family lipoproteins. Nucleotide sequencing analysis of the pepIMP13 gene region revealed that the promoter-containing 135-bp DNA of this gene had the structure of an invertible element that functioned as a switch for gene expression. In addition, all of the mpl genes of M. penetrans HF-2 were identified using the whole-genome sequence data that has recently become available for this bacterium. There are at least 38 mpl genes in the M. penetrans HF-2 genome. Interestingly, most of these mpl genes possess invertible promoter-like sequences, similar to those of the pepIMP13 gene promoter. A model for the generation of surface antigenic variation by multiple promoter inversions is proposed.
机译：支原体支链是支原体属的新近鉴定种。首先从感染了人类免疫缺陷病毒（HIV）的患者的尿液样本中分离出来。 M。 penetrans 会频繁改变其表面抗原分布。这些变化源自表面膜脂蛋白P35家族的ON→OFF相变。 P35家族脂蛋白是M期人类免疫系统识别的主要抗原。 penetrans 感染，并由 mpl 基因编码。 P35家族脂蛋白的相变发生在 mpl 基因的转录水平上。但是，确切的遗传机制尚不清楚。在这项研究中，表面抗原谱在 M中改变的分子机制。对penetrans 进行了调查。重点是存在于 M中的46 kDa蛋白。 penetrans 菌株HF-2，但不在GTU型菌株中。 46 kDa蛋白是先前报道的 mpl 基因pepIMP13的产物，其氨基酸末端序列与P35家族脂蛋白相同。对pepIMP13基因区域的核苷酸测序分析表明，该基因的含启动子的135 bp DNA具有可逆元件的结构，该元件可作为基因表达的开关。另外， M的所有 mpl 基因。利用最近可用于该细菌的全基因组序列数据鉴定了penetrans HF-2。 M中至少有38个 mpl 基因。 penetrans HF-2基因组。有趣的是，大多数这些 mpl 基因都具有类似于pepIMP13基因启动子的可逆启动子样序列。提出了通过多个启动子倒置产生表面抗原变异的模型。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号