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首页> 外文期刊>Journal of bacteriology >A Rac Homolog Is Required for Induction of Hyphal Growth in the Dimorphic Yeast Yarrowia lipolytica
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A Rac Homolog Is Required for Induction of Hyphal Growth in the Dimorphic Yeast Yarrowia lipolytica

机译：Rac同源物是诱导双态酵母解脂耶氏酵母中菌丝生长所必需的。

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Dimorphism in fungi is believed to constitute a mechanism of response to adverse conditions and represents an important attribute for the development of virulence by a number of pathogenic fungal species. We have isolated YlRAC1, a gene encoding a 192-amino-acid protein that is essential for hyphal growth in the dimorphic yeast Yarrowia lipolytica and which represents the first Rac homolog described for fungi. YlRAC1 is not an essential gene, and its deletion does not affect the ability to mate or impair actin polarization in Y. lipolytica. However, strains lacking functional YlRAC1 show alterations in cell morphology, suggesting that the function of YlRAC1 may be related to some aspect of the polarization of cell growth. Northern blot analysis showed that transcription of YlRAC1 increases steadily during the yeast-to-hypha transition, while Southern blot analysis of genomic DNA suggested the presence of severalRAC family members in Y. lipolytica. Interestingly, strains lacking functional YlRAC1 are still able to grow as the pseudohyphal form and to invade agar, thus pointing to a function for YlRAC1 downstream of MHY1, a previously isolated gene encoding a C2H2-type zinc finger protein with the ability to bind putative stress response elements and whose activity is essential for both hyphal and pseudohyphal growth in Y. lipolytica.
机译：真菌中的双态性被认为构成了对不利条件的反应机制，并且代表了许多致病性真菌物种致病力发展的重要属性。我们已经分离出 YlRAC1 ，该基因编码一个192个氨基酸的蛋白，该蛋白对于双态酵母 lipolytica 中的菌丝生长至关重要，它代表了第一个描述的Rac同源物菌类。 YlRAC1 不是必需的基因，其缺失不影响 Y中肌动蛋白极化的交配或损害能力。解脂脂。然而，缺乏功能性 YlRAC1 的菌株表现出细胞形态的改变，这表明 YlRAC1 的功能可能与细胞生长极化的某些方面有关。 Northern印迹分析表明，在酵母菌到菌丝的过渡过程中， YlRAC1 的转录稳定增加，而对基因组DNA的Southern印迹分析表明， RAC 家族中有多个 RAC 家族成员。 em> Y。解脂脂。有趣的是，缺乏功能性 YlRAC1 的菌株仍能够以假菌丝形式生长并侵入琼脂，因此指向 MHY1 下游的 YlRAC1 的功能。 >，一种先前分离的编码C 2 H 2 型锌指蛋白的基因，具有结合假定的应激反应元件的能力，并且其活性对于菌丝和假菌丝都是必不可少的Y的增长。解脂脂。

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