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首页> 外文期刊>Journal of bacteriology >The Linear Double-Stranded DNA of Phage Bam35 Enters Lysogenic Host Cells, but the Late Phage Functions Are Suppressed
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The Linear Double-Stranded DNA of Phage Bam35 Enters Lysogenic Host Cells, but the Late Phage Functions Are Suppressed

机译：噬菌体Bam35的线性双链DNA进入溶原性宿主细胞，但晚期噬菌体功能受到抑制。

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Bam35, a temperate double-stranded DNA bacteriophage with a 15-kb linear genome, infects gram-positive Bacillus thuringiensis cells. Bam35 morphology and genome organization resemble those of PRD1, a lytic phage infecting gram-negative bacteria. Bam35 and PRD1 have an outer protein coat surrounding a membrane that encloses the viral DNA. We used electrochemical methods to investigate physiological changes of the lysogenic and nonlysogenic hosts during Bam35 DNA entry and host cell lysis. During viral DNA entry, there was an early temporal decrease of membrane voltage associated with K+ efflux that took place when either lysogenic or nonlysogenic hosts were infected. Approximately 40 min postinfection, a second strong K+ efflux was registered that was proposed to be associated with the insertion of holin molecules into the plasma membrane. This phenomenon occurred only when nonlysogenic cells were infected. Lysogenic hosts rarely were observed entering the lytic cycle as demonstrated by thin-section electron microscopy.
机译：Bam35是一种具有15kb线性基因组的温带双链DNA噬菌体，可感染革兰氏阳性苏云金芽孢杆菌细胞。 Bam35的形态和基因组组织类似于PRD1，PRD1是一种裂解性噬菌体，感染革兰氏阴性细菌。 Bam35和PRD1在包裹病毒DNA的膜周围有一层蛋白外层。我们使用电化学方法研究Bam35 DNA进入和宿主细胞裂解过程中溶源性和非溶源性宿主的生理变化。在病毒DNA进入过程中，感染溶源性或非溶源性宿主时，与K + 外流相关的膜电压会在早期出现下降。感染后大约40分钟，记录到第二次强K + 外排，这被认为与将holin分子插入质膜有关。仅当感染了非溶源性细胞时，才会发生此现象。薄层电子显微镜显示，很少观察到溶源性宿主进入裂解周期。

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