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首页> 外文期刊>Journal of bacteriology >A stationary-phase gene in Saccharomyces cerevisiae is a member of a novel, highly conserved gene family.
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A stationary-phase gene in Saccharomyces cerevisiae is a member of a novel, highly conserved gene family.

机译：酿酒酵母中的固定相基因是一个新的，高度保守的基因家族的成员。

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The regulation of cellular growth and proliferation in response to environmental cues is critical for development and the maintenance of viability in all organisms. In unicellular organisms, such as the budding yeast Saccharomyces cerevisiae, growth and proliferation are regulated by nutrient availability. We have described changes in the pattern of protein synthesis during the growth of S. cerevisiae cells to stationary phase (E. K. Fuge, E. L. Braun, and M. Werner-Washburne, J. Bacteriol. 176:5802-5813, 1994) and noted a protein, which we designated Snz1p (p35), that shows increased synthesis after entry into stationary phase. We report here the identification of the SNZ1 gene, which encodes this protein. We detected increased SNZ1 mRNA accumulation almost 2 days after glucose exhaustion, significantly later than that of mRNAs encoded by other postexponential genes. SNZ1-related sequences were detected in phylogenetically diverse organisms by sequence comparisons and low-stringency hybridization. Multiple SNZ1-related sequences were detected in some organisms, including S. cerevisiae. Snz1p was found to be among the most evolutionarily conserved proteins currently identified, indicating that we have identified a novel, highly conserved protein involved in growth arrest in S. cerevisiae. The broad phylogenetic distribution, the regulation of the SNZ1 mRNA and protein in S. cerevisiae, and identification of a Snz protein modified during sporulation in the gram-positive bacterium Bacillus subtilis support the hypothesis that Snz proteins are part of an ancient response that occurs during nutrient limitation and growth arrest.
机译：响应环境线索而调节细胞生长和增殖对于所有生物的发育和维持生存力至关重要。在单细胞生物中，例如出芽的酵母酿酒酵母，其生长和增殖受养分利用率的调节。我们已经描述了在酿酒酵母细胞生长到固定相期间蛋白质合成模式的变化（EK Fuge，EL Braun和M. Werner-Washburne，J。Bacteriol。176：5802-5813，1994），并指出了我们称其为Snz1p（p35）的蛋白质，在进入固定相后显示出增加的合成。我们在这里报告了编码该蛋白的SNZ1基因的鉴定。我们在葡萄糖耗尽后将近2天检测到SNZ1 mRNA的积累增加，显着晚于其他指数后基因编码的mRNA的积累。通过序列比较和低严格性杂交，在系统发育多样的生物中检测到SNZ1相关序列。在包括酿酒酵母在内的某些生物中检测到多个SNZ1相关序列。 Snz1p被发现是目前鉴定出的进化最保守的蛋白质之一，这表明我们已经鉴定出一种与酿酒酵母生长停滞有关的新型，高度保守的蛋白质。广泛的系统发育分布，酿酒酵母中SNZ1 mRNA和蛋白的调控以及革兰氏阳性枯草芽孢杆菌芽孢形成过程中修饰的Snz蛋白的鉴定支持以下假设：Snz蛋白是古老的反应过程中发生的一部分营养限制和生长停滞。

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