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首页> 外文期刊>Journal of bacteriology >Suppression of mutant phenotypes of the Agrobacterium tumefaciens VirB11 ATPase by overproduction of VirB proteins.
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Suppression of mutant phenotypes of the Agrobacterium tumefaciens VirB11 ATPase by overproduction of VirB proteins.

机译：通过过量生产VirB蛋白来抑制根癌农杆菌VirB11 ATPase的突变表型。

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The Agrobacterium tumefaciens VirB11 ATPase is postulated to assemble with VirB proteins and the VirD4 protein into a transport system which is dedicated to the export of oncogenic nucleoprotein particles to plant cells. To gain genetic evidence for interactions between VirB11 and other subunits of this transport system, we screened a PCR-mutagenized virB11 library for alleles that diminish the virulence of the wild-type strain A348. Two classes of alleles displaying negative dominance were identified. One class failed to complement a delta virB11 mutation, indicating that the corresponding mutant proteins are nonfunctional. The second class complemented the delta virB11 mutation, indicating that the mutant proteins are fully functional in strains devoid of native VirB11. Mutations of both classes of alleles were in codons for residues clustered in two regions of VirB11, both located outside the Walker A nucleotide binding motif. All dominant alleles were suppressed at least to some extent by multicopy expression of the virB9, virB10, and/or virB11 genes. Taken together, results of these investigations indicate that (i) a functional T-complex transporter is composed of more than one VirB11 subunit and (ii) VirB11 undergoes complex formation with VirB9 and VirB10 during transporter biogenesis.
机译：根癌土壤杆菌的VirB11 ATPase被假定与VirB蛋白和VirD4蛋白组装成一个运输系统，该系统专门用于将致癌核蛋白颗粒输出至植物细胞。为了获得有关VirB11和该转运系统其他亚基之间相互作用的遗传证据，我们筛选了PCR诱变的virB11文库，寻找减少野生型菌株A348毒力的等位基因。确定了两类显示负优势的等位基因。一类未能补充del virB11突变，表明相应的突变蛋白无功能。第二类是对增量virB11突变的补充，表明该突变蛋白在没有天然VirB11的菌株中具有完全功能。这两类等位基因的突变都存在于位于VirB11的两个区域中的残基的密码子中，两个区域均位于Walker A核苷酸结合基序之外。 virB9，virB10和/或virB11基因的多拷贝表达至少抑制了所有显性等位基因。综上所述，这些研究的结果表明（i）功能性T-复合转运蛋白由一个以上的VirB11亚基组成，（ii）VirB11在转运蛋白的生物发生过程中与VirB9和VirB10形成复合物。

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