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首页> 外文期刊>Journal of bacteriology >Cloning, Expression, and Catabolite Repression of a Gene Encoding β-Galactosidase of Bacillus megateriumATCC 14581
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Cloning, Expression, and Catabolite Repression of a Gene Encoding β-Galactosidase of Bacillus megateriumATCC 14581

机译:巨大芽孢杆菌β-半乳糖苷酶编码基因ATCC 14581的克隆,表达及分解代谢抑制

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A gene encoding β-galactosidase, designated mbgA, was isolated from Bacillus megaterium ATCC 14581. Chromosomal β-galactosidase production could be dramatically induced by lactose but not by isopropyl-β-d-thiogalactopyranoside (IPTG) and was subject to catabolite repression by glucose. Disruption ofmbgA in the B. megaterium chromosome resulted in loss of lactose-inducible β-galactosidase production. A 27-bp inverted repeat was found to overlap the mbgA promoter sequence. Two partially overlapping catabolite-responsive elements (CREs) were identified within the inverted repeat. Base substitutions within CRE-I and/or CRE-II caused partial relief from catabolite repression. The results suggest that the 27-bp inverted repeat may serve as a target for a catabolite repressor(s).
机译:从巨大芽孢杆菌ATCC 14581中分离到一个编码β-半乳糖苷酶的基因,命名为 mbgA 。乳糖可以显着诱导染色体β-半乳糖苷酶的产生,而异丙基-β-则不能。 d-硫代半乳糖吡喃糖苷(IPTG)受到葡萄糖的分解代谢物抑制。 B中的 mbgA 中断。巨型染色体会导致乳糖诱导的β-半乳糖苷酶产生的损失。发现一个27 bp的反向重复序列与 mbgA 启动子序列重叠。在反向重复序列中鉴定出两个部分重叠的分解代谢物响应元件(CRE)。 CRE-I和/或CRE-II中的碱基取代导致部分分解代谢物阻抑。结果表明,27 bp的反向重复序列可以用作分解代谢物阻遏物的靶标。

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