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首页> 外文期刊>Journal of bacteriology >Intracellular Glucose Concentration in Derepressed Yeast Cells Consuming Glucose Is High Enough To Reduce the Glucose Transport Rate by 50%
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Intracellular Glucose Concentration in Derepressed Yeast Cells Consuming Glucose Is High Enough To Reduce the Glucose Transport Rate by 50%

机译：消耗葡萄糖的低阻酵母细胞中的细胞内葡萄糖浓度高到足以将葡萄糖转运速率降低50％

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摘要

In Saccharomyces cerevisiae cells exhibiting high-affinity glucose transport, the glucose consumption rate at extracellular concentrations above 10 mM was only half of the zerotrans-influx rate. To determine if this regulation of glucose transport might be a consequence of intracellular free glucose we developed a new method to measure intracellular glucose concentrations in cells metabolizing glucose, which compares glucose stereoisomers to correct for adhering glucose. The intracellular glucose concentration was 1.5 mM, much higher than in most earlier reports. We show that for the simplest model of a glucose carrier, this concentration is sufficient to reduce the glucose influx by 50%. We conclude that intracellular glucose is the most likely candidate for the observed regulation of glucose import and hence glycolysis. We discuss the possibility that intracellular glucose functions as a primary signal molecule in these cells.
机译：在表现出高亲和力葡萄糖转运的酿酒酵母细胞中，细胞外浓度高于10 mM时的葡萄糖消耗速率仅为零 trans 流入速率的一半。为了确定这种葡萄糖转运的调节是否可能是细胞内游离葡萄糖的结果，我们开发了一种测量代谢葡萄糖的细胞中细胞内葡萄糖浓度的新方法，该方法比较了葡萄糖立体异构体以纠正粘附的葡萄糖。细胞内葡萄糖浓度为1.5 mM，远高于大多数早期报道。我们表明，对于最简单的葡萄糖载体模型，该浓度足以减少50％的葡萄糖流入。我们得出结论，细胞内葡萄糖是观察到的葡萄糖进口调节和糖酵解调节的最可能候选者。我们讨论了细胞内葡萄糖在这些细胞中充当主要信号分子的可能性。

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