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首页> 外文期刊>Journal of bacteriology >Recovery of DNA Replication in UV-IrradiatedEscherichia coli Requires both Excision Repair and RecF Protein Function
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Recovery of DNA Replication in UV-IrradiatedEscherichia coli Requires both Excision Repair and RecF Protein Function

机译：在紫外线照射的大肠杆菌中恢复DNA复制需要切除修复和RecF蛋白功能

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After UV doses that disrupt DNA replication, the recovery of replication at replication forks in Escherichia colirequires a functional copy of the recF gene. InrecF mutants, replication fails to recover and extensive degradation of the nascent DNA occurs, suggesting that recFfunction is needed to stabilize the disrupted replication forks and facilitate the process of recovery. We show here that the ability ofrecF to promote the recovery of replication requires that the disrupting lesions be removed. In the absence of excision repair,recF + cells protect the nascent DNA at replication forks, but replication does not resume. The classical view is that recombination proteins operate in pathways that are independent from DNA repair, and therefore the functions of Rec proteins have been studied in repair-deficient cells. However, mutations in eitheruvr or recF result in failure to recover replication at UV doses from which wild-type cells recover efficiently, suggesting that recF and excision repair contribute to a common pathway in the recovery of replication.
机译：在紫外线剂量破坏了DNA复制后，大肠杆菌中复制叉处的复制恢复需要 recF 基因的功能性拷贝。在 recF 突变体中，复制无法恢复并且新生DNA发生了广泛的降解，这表明需要 recF 功能来稳定被破坏的复制叉并促进恢复过程。我们在这里显示 recF 促进复制恢复的能力要求去除破坏性病变。在没有切除修复的情况下， recF + 细胞在复制叉处保护新生的DNA，但复制不会恢复。经典观点是重组蛋白以与DNA修复无关的途径运作，因此，在修复缺陷型细胞中研究了Rec蛋白的功能。但是， uvr 或 recF 中的突变导致无法以紫外线剂量恢复复制，野生型细胞可从紫外线剂量有效地恢复，这表明 recF 切除修复是复制恢复中的共同途径。

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