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首页> 外文期刊>Journal of bacteriology >mucK, a gene in Acinetobacter calcoaceticus ADP1 (BD413), encodes the ability to grow on exogenous cis,cis-muconate as the sole carbon source.
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mucK, a gene in Acinetobacter calcoaceticus ADP1 (BD413), encodes the ability to grow on exogenous cis,cis-muconate as the sole carbon source.

机译：mucK是钙乙酸不动杆菌ADP1（BD413）中的一个基因，具有在外源顺式，顺式-粘液酸作为唯一碳源上生长的能力。

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Benzyl alcohol, benzaldehyde, benzoate, and anthranilate are metabolized via catechol, cis,cis-muconate, and the beta-ketoadipate pathway in Acinetobacter calcoaceticus ADP1 (BD413). Mutant strain ISA25 with a deletion spanning catBCIJF and unable to metabolize muconate further will not grow in the presence of an aromatic precursor of muconate. Growth on fumarate as the sole carbon source with added benzyl alcohol or benzaldehyde selected spontaneous mutants of ISA25. After repair of the cat deletion by natural transformation with linearized plasmid pPAN4 (catBCIJF) 10 mutants were unable to grow on benzoate of cis,cis-muconate but could still grow on anthranilate. Transformation with wild-type chromosomal DNA demonstrated the presence of two unlinked mutations in each strain, one in the benABCD region, encoding the conversion of benzoate to catechol, and the other in a gene determining the ability to grow on exogenous cis,cis-muconate. The wild-type gene, named mucK, was cloned into pUC18, and its nucleotide sequence was determined. It encodes a 413-residue protein of M(r) = 45,252 which is a member of a superfamily of membrane transport proteins and which is within a subgroup involved in the uptake of organic acids. Five of the mutant alleles were cloned, and the mutations were determined by nucleotide sequencing. All the mutations were in the mucK coding region and consisted of three deletions, one duplication, and a substitution. Insertional inactivation of mucK resulted in the loss of the ability to utilize exogenous muconate. The location of mucK on the chromosome appeared to be unique for genes associated with the benzoate branch of the beta-ketoadipate pathway in being close to the pca-qui-pob gene cluster (for p-hydroxybenzoate utilization) and distant from the functionally related ben-cat cluster. Downstream of mucK and transcribed in the same direction is an open reading frame encoding a protein of 570 residues (M(r) = 63,002) which shows considerable homology with a mammalian electron transport protein; its insertional inactivation had no detectable phenotypic effect.
机译：苯甲醇，苯甲醛，苯甲酸酯和邻氨基苯甲酸酯通过儿茶酚，顺式，顺式-粘康酸酯和β-酮己二酸酯途径在钙乙酸不动杆菌ADP1（BD413）中代谢。在存在粘液的芳香族前体的情况下，具有跨越catBCIJF的缺失且无法进一步代谢粘液的突变株ISA25将不会生长。富马酸盐作为唯一碳源的生长，添加苯甲醇或苯甲醛可以选择ISA25的自发突变体。用线性化质粒pPAN4（catBCIJF）通过自然转化修复了猫的缺失后，有10个突变体无法在顺式，顺式-粘康酸酯的苯甲酸盐上生长，但仍可以在邻氨基苯甲酸上生长。用野生型染色体DNA进行的转化证明，每个菌株中均存在两个未连锁的突变，一个在beABABCD区，编码苯甲酸酯向邻苯二酚的转化，另一个在决定外源顺式，顺式粘液生长能力的基因中。将野生型基因mucK克隆到pUC18中，并确定其核苷酸序列。它编码一个413个残基的蛋白，M（r）= 45,252，它是膜转运蛋白超家族的成员，并且在一个参与吸收有机酸的亚组内。克隆了五个突变等位基因，并通过核苷酸测序确定了突变。所有的突变都在mucK编码区，由三个缺失，一个重复和一个取代组成。 mucK的插入失活导致失去利用外源粘液的能力。 mucK在染色体上的位置对于与β-酮己二酸途径的苯甲酸酯分支相关的基因而言似乎是唯一的，因为它靠近pca-qui-pob基因簇（用于p-羟基苯甲酸酯利用）并且与功能相关的ben远离-cat群集。 mucK的下游并沿相同方向转录是一个开放阅读框，其编码一个570个残基的蛋白质（M（r = 63,002）），与哺乳动物的电子转运蛋白具有相当的同源性。其插入失活没有可检测的表型作用。

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