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首页> 外文期刊>Journal of bacteriology >Multiple Roles for TnpI Recombinase in Regulation of Tn5401 Transposition in Bacillus thuringiensis
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Multiple Roles for TnpI Recombinase in Regulation of Tn5401 Transposition in Bacillus thuringiensis

机译:TnpI重组酶在苏云金芽胞杆菌中Tn5401转座调控中的多重作用。

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Tn5401 is a class II transposable element derived from the gram-positive bacterium Bacillus thuringiensis. The 4,837-bp transposon encodes a Tn3-like transposase (TnpA) and an integrase-like recombinase (TnpI) and is notable for its unusually long 53-bp terminal inverted repeats (TIRs). ThetnpA and tnpI genes are transcribed from a common promoter, designated PR, that is subject to negative regulation by TnpI. The TIRs of Tn5401 each contain a 38-bp sequence that can be aligned with the 38- to 40-bp TIR sequences of Tn3-like transposons and an adjacent 12-bp sequence that binds TnpI. This unique juxtaposition of TnpA and TnpI binding sites suggests that TnpI may regulate the binding or catalytic activity of TnpA. The results of the present study indicate that TnpI, in addition to functioning as a site-specific recombinase and as a transcriptional repressor, is required for TnpA binding to the TIRs of Tn5401.
机译:Tn 5401 是源自革兰氏阳性细菌苏云金芽孢杆菌的II类转座因子。 4,837 bp的转座子编码Tn 3 样转座酶(TnpA)和整合酶样重组酶(TnpI),并因其异常长的53 bp末端反向重复序列(TIR)而著称。 tnpA tnpI 基因是从共同的启动子P R 转录而成的,该启动子受TnpI的负调控。 Tn 5401 的TIR各自包含一个38 bp的序列,可以与Tn 3 样转座子的38到40 bp的TIR序列比对,并且相邻的12个结合TnpI的-bp序列。 TnpA和TnpI结合位点的这种独特的并置表明TnpI可以调节TnpA的结合或催化活性。本研究的结果表明,TnpI除了起位点特异性重组酶和转录阻遏物的作用外,还需要TnpA与Tn 5401 的TIR结合。

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