...
首页> 外文期刊>Journal of bacteriology >The torYZ (yecK bisZ) Operon Encodes a Third Respiratory Trimethylamine N-Oxide Reductase inEscherichia coli
【24h】

The torYZ (yecK bisZ) Operon Encodes a Third Respiratory Trimethylamine N-Oxide Reductase inEscherichia coli

机译:torYZ(yecK bisZ)操纵子编码大肠杆菌中的第三种呼吸性三甲胺N-氧化物还原酶

获取原文
   

获取外文期刊封面封底 >>

       

摘要

The bisZ gene of Escherichia coli was previously described as encoding a minor biotin sulfoxide (BSO) reductase in addition to the main cytoplasmic BSO reductase, BisC. In this study, bisZ has been renamed torZ based on the findings that (i) the torZ gene product, TorZ, is able to reduce trimethylamine N-oxide (TMAO) more efficiently than BSO; (ii) although TorZ is more homologous to BisC than to the TMAO reductase TorA (63 and 42% identity, respectively), it is located mainly in the periplasm as is TorA; (iii)torZ belongs to the torYZ operon, and the first gene, torY (formerly yecK), encodes a pentahemic c-type cytochrome homologous to the TorC cytochrome of the TorCAD respiratory system. Furthermore, the torYZ operon encodes a third TMAO respiratory system, with catalytic properties that are clearly different from those of the TorCAD and the DmsABC systems. ThetorYZ and the torCAD operons may have diverged from a common ancestor, but, surprisingly, notorD homologue is found in the sequences aroundtorYZ. Moreover, the torYZ operon is expressed at very low levels under the conditions tested, and, in contrast to torCAD, it is not induced by TMAO or dimethyl sulfoxide.
机译:以前,大肠杆菌 bisZ 基因除了主要的胞质BSO还原酶BisC外,还编码一种次生物素亚砜(BSO)还原酶。在这项研究中,基于以下发现, bisZ 被重命名为 torZ (i) torZ 基因产物TorZ能够还原三甲胺 N -氧化物(TMAO)比BSO更有效; (ii)尽管TorZ与BisC的同源性比与TMAO还原酶TorA的同源性更高(分别为63%和42%),但它与TorA一样主要位于周质中; (iii) torZ 属于 torYZ 操纵子,第一个基因 torY (以前称为 yecK )编码与TorCAD呼吸系统的TorC细胞色素同源的五元 c 型细胞色素。此外, torYZ 操纵子编码第三种TMAO呼吸系统,其催化特性与TorCAD和DmsABC系统明显不同。 torYZ torCAD 操纵子可能与共同祖先不同,但是令人惊讶的是,在附近没有发现 torD 同源物> torYZ 。此外,在测试条件下, torYZ 操纵子的表达水平非常低,与 torCAD 相比,它不是由TMAO或二甲基亚砜诱导的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号