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首页> 外文期刊>Journal of bacteriology >The torR gene of Escherichia coli encodes a response regulator protein involved in the expression of the trimethylamine N-oxide reductase genes.
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The torR gene of Escherichia coli encodes a response regulator protein involved in the expression of the trimethylamine N-oxide reductase genes.

机译：大肠杆菌的torR基因编码一个响应调节蛋白，参与三甲胺N-氧化物还原酶基因的表达。

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摘要

Expression of the Escherichia coli torCAD operon encoding the trimethylamine N-oxide (TMAO) reductase system is induced by both TMAO and anaerobiosis. A torR insertion mutant unable to express the torA gene had previously been isolated. The torR gene was cloned and sequenced. It encodes a 25,000-Da protein which shares homology with response regulators of two-component systems and belongs to the OmpR-PhoB subclass. Overproduction of TorR mimics the presence of the inducer TMAO while the anaerobic control is unchanged, suggesting that TorR mediates only the TMAO induction. The overproduced TorR protein was purified to more than 90%. The torR gene is located just upstream of the torCAD operon, with an opposite transcription direction. The torR-torCAD intergenic region is unusual in that it contains four direct repeats of a 10-nucleotide motif. Part or all of these motifs could be involved in the binding of TorR. The gene encoding the sensor partner does not seem to be adjacent to torR, since the divergent open reading frame found immediately downstream of torR exhibits none of the features of a protein histidine kinase.
机译：编码三甲胺N-氧化物（TMAO）还原酶系统的大肠杆菌torCAD操纵子的表达是由TMAO和厌氧菌诱导的。先前已经分离出不能表达torA基因的torR插入突变体。克隆了torR基因并进行了测序。它编码一个25,000-Da的蛋白质，该蛋白质与两组分系统的反应调节因子具有同源性，属于OmpR-PhoB子类。 TorR的过量生产模拟了诱导剂TMAO的存在，而厌氧控制未改变，表明TorR仅介导TMAO诱导。过量生产的TorR蛋白被纯化至90％以上。 torR基因位于torCAD操纵子的上游，转录方向相反。 torR-torCAD基因间区域不寻常，因为它包含10个核苷酸基序的四个直接重复。这些基序的部分或全部可能与TorR的结合有关。编码传感器伴侣的基因似乎并不与torR相邻，因为紧接在torR下游的发散的开放阅读框没有蛋白组氨酸激酶的任何特征。

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