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首页> 外文期刊>Journal of bacteriology >Mutation of ndh Genes Leads to Inhibition of CO2 Uptake Rather than HCO3 ?Uptake in Synechocystis sp. Strain PCC 6803
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Mutation of ndh Genes Leads to Inhibition of CO2 Uptake Rather than HCO3 ?Uptake in Synechocystis sp. Strain PCC 6803

机译：ndh基因的突变导致抑制集胞藻属物种中的CO2吸收而不是HCO3吸收。应变PCC 6803

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Six mutants (B1 to B6) that grew poorly in air on BG11 agar plates buffered at pH 8.0 were rescued after mutations were introduced intondhB of wild-type (WT) Synechocystis sp. strain PCC 6803. In these mutants and a mutant (M55) lacking ndhB, CO2 uptake was much more strongly inhibited than HCO3 ? uptake, i.e., the activities of CO2 and HCO3 ? uptake in B1 were 9 and 85% of those in the WT, respectively. Most of the mutants grew very slowly or did not grow at all at pH 6.5 or 7.0 in air, and their ability to grow under these conditions was correlated with CO2 uptake capacity. Detailed studies of B1 and M55 indicated that the mutants grew as fast as the WT in liquid at pH 8.0 under air, although they grew poorly on agar plates. The contribution of CO2 uptake appears to be larger on solid medium. Five mutants were constructed by inactivating each of the fivendhD genes in Synechocystis sp. strain PCC 6803. The mutant lacking ndhD3 grew much more slowly than the WT at pH 6.5 under 50 ppm CO2, although otherndhD mutants grew like the WT under these conditions and showed low affinity for CO2 uptake. These results indicated the presence of multiple NAD(P)H dehydrogenase type I complexes with specific roles.
机译：将突变引入野生型（ Synechocystis）的 ndhB 后，拯救了六个在pH 8.0缓冲的BG11琼脂平板上在空气中生长较差的突变体（B1至B6）。 sp。菌株PCC6803。在这些突变体和缺少 ndhB 的突变体（M55）中，对CO 2 的摄取比对HCO 3 ？摄取，即B1中CO 2 和HCO 3 ？摄取的活性分别为9和85％分别在WT中。大多数突变体在空气中的pH值为6.5或7.0时生长非常缓慢或根本不生长，并且在这些条件下的生长能力与CO 2 的吸收能力相关。 B1和M55的详细研究表明，在空气中pH 8.0的条件下，突变体的生长速度与WT一样快，尽管它们在琼脂平板上生长较差。在固体培养基上，CO 2 摄取的贡献似乎更大。通过使 Synechocystis sp。的五个 ndhD 基因中的每一个失活，构建了五个突变体。在50 ppm CO 2 下，缺乏 ndhD3 的突变体在pH 6.5的情况下比WT生长慢得多，尽管其他 ndhD 突变体也在生长在这种条件下，WT表现出对CO 2 吸收的低亲和力。这些结果表明存在多个具有特定作用的NAD（P）H脱氢酶I型复合物。

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