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首页> 外文期刊>Journal of bacteriology >Identification and Characterization of a Developmentally Regulated Protein, EshA, Required for Sporogenic Hyphal Branches in Streptomyces griseus
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Identification and Characterization of a Developmentally Regulated Protein, EshA, Required for Sporogenic Hyphal Branches in Streptomyces griseus

机译：鉴定和表征灰链霉菌中致孢菌菌丝分支所需的发育调控蛋白EshA。

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To identify sporulation-specific proteins that might serve as targets of developmental regulatory factors inStreptomyces, we examined total proteins ofStreptomyces griseus by two-dimensional gel electrophoresis. Among five proteins that were present at high levels during sporulation but absent from vegetative cells, two of the proteins, P3 and P4, were absent from developmental mutants that undergo aberrant morphogenesis. The deduced amino acid sequence of the gene that encodes P3 (EshA) showed extensive similarity to proteins from mycobacteria and a cyanobacterium, Synechococcus, that are abundant during nutritional stress but whose functions are unknown. Uniquely among these proteins, EshA contains a cyclic nucleotide-binding domain, suggesting that the activity of EshA may be modulated by a cyclic nucleotide. The eshA gene was strongly expressed from a single transcription start site only during sporulation, and accumulation of the eshA transcript depended on a developmental gene, bldA. During submerged sporulation, a null mutant strain that produced no EshA could not extend sporogenic hyphae from new branch points but instead accelerated septation and spore maturation at the preexisting vegetative filaments. These results indicated that EshA is required for the growth of sporogenic hyphae and localization of septation and spore maturation but not for spore viability.
机译：为了鉴定可能是链霉菌中发育调控因子的靶标的孢子特异性蛋白，我们通过二维凝胶电泳检查了 Streptomyces griseus 的总蛋白。在孢子形成过程中高水平存在但营养细胞中不存在的五种蛋白质中，发生异常形态发生的发育突变体中不存在两种蛋白质P3和P4。推导的编码P3（EshA）的基因的氨基酸序列与分枝杆菌和蓝细菌 Synechococcus 的蛋白质具有广泛的相似性，这些蛋白质在营养压力下很丰富，但功能未知。在这些蛋白质中，EshA独特地包含一个环状核苷酸结合结构域，这表明EshA的活性可能受到环状核苷酸的调节。 eshA 基因仅在孢子形成期间从单个转录起始位点强烈表达，而 eshA 转录本的积累取决于发育基因 bldA 。在淹没的孢子形成过程中，没有产生EshA的无效突变株不能从新的分支点延伸孢子菌丝，而是在先前存在的营养丝上加速分离和孢子成熟。这些结果表明，EshA是孢子丝菌丝的生长以及分离和芽孢成熟的定位所必需的，而不是孢子生存力所必需的。

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