Defense against Protein Carbonylation by DnaK/DnaJ and Proteases of the Heat Shock Regulon

?sa Fredriksson; Manuel Ballesteros; Sam Dukan; Thomas Nystr?m

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Defense against Protein Carbonylation by DnaK/DnaJ and Proteases of the Heat Shock Regulon

机译：DnaK / DnaJ和热休克调节蛋白对蛋白质羰基化的防御作用

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Protein carbonylation is an irreversible oxidative modification that increases during organism aging and bacterial growth arrest. We analyzed whether the heat shock regulon has a role in defending Escherichia coli cells against this deleterious modification upon entry into stationary phase. Providing the cell with ectopically elevated levels of the heat shock transcription factor, σ32, effectively reduced stasis-induced carbonylation. Separate overproduction of the major chaperone systems, DnaK/DnaJ and GroEL/GroES, established that the former of these is more important in counteracting protein carbonylation. Deletion of the heat shock proteases Lon and HslVU enhanced carbonylation whereas a clpP deletion alone had no effect. However, ClpP appears to have a role in reducing protein carbonyls in cells lacking Lon and HslVU. Proteomic immunodetection of carbonylated proteins in the wild-type, lon, and hslVU strains demonstrated that the same spectrum of proteins displayed a higher load of carbonyl groups in the lon and hslVU mutants. These proteins included the β-subunit of RNA polymerase, elongation factors Tu and G, the E1 subunit of the pyruvate dehydrogenase complex, isocitrate dehydrogenase, 6-phosphogluconate dehydrogenase, and serine hydroxymethyltranferase.

机译：蛋白质羰基化是不可逆的氧化修饰，在生物衰老和细菌生长停滞期间会增加。我们分析了进入休克期后，热休克调节子是否在防御大肠杆菌细胞免受这种有害修饰的作用。为细胞提供异位升高的热激转录因子σ 32 可以有效地减少瘀滞诱导的羰基化。主要的分子伴侣系统DnaK / DnaJ和GroEL / GroES的单独过量生产已确定，前者在抵消蛋白质羰基化作用方面更为重要。热休克蛋白酶Lon和HslVU的缺失增强了羰基化作用，而单独的 clpP 缺失则没有作用。但是，ClpP似乎在减少缺乏Lon和HslVU的细胞中减少蛋白质羰基的作用。蛋白质组免疫检测野生株， lon 和 hslVU 菌株中的羰基化蛋白表明，相同光谱的蛋白质在 lon中显示出更高的羰基负载量和 hslVU 突变体。这些蛋白质包括RNA聚合酶的β亚基，延伸因子Tu和G，丙酮酸脱氢酶复合物的E1亚基，异柠檬酸脱氢酶，6-磷酸葡萄糖酸脱氢酶和丝氨酸羟甲基转移酶。 展开▼

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《Journal of bacteriology》 |2005年第12期|共7页

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?sa Fredriksson; Manuel Ballesteros; Sam Dukan; Thomas Nystr?m;
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中图分类微生物学;

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专利

1. The role of DnaK/DnaJ and GroEL/GroES systems in the removal of endogenous proteins aggregated by heat‐shock from Escherichia coli cells [J] . K#x119, dzierska S., Staniszewska M., FEBS Letters . 1999,第2a3期

机译：DnaK / DnaJ和GroEL / GroES系统在去除热休克从大肠杆菌细胞中聚集的内源蛋白中的作用

2. Temperature-controlled activity of DnaK-DnaJ-GrpE chaperones: Protein-folding arrest and recovery during and after heat shock depends on the substrate protein and the GrpE concentration [J] . Diamant S., Goloubinoff P. Biochemistry . 1998,第27期

机译：DnaK-DnaJ-GrpE分子伴侣的温度控制活性：热休克期间和之后的蛋白质折叠阻滞和恢复取决于底物蛋白质和GrpE浓度

3. Heat shock proteins DnaJ, DnaK, and GrpE stimulate P1 plasmid replication by promoting initiator binding to the origin. [J] . S Sozhamannan, D K Chattoraj Journal of bacteriology . 1993,第11期

机译：热休克蛋白DnaJ，DnaK和GrpE通过促进启动子与起点的结合来刺激P1质粒复制。

4. Structural Bioinformatic Approach to Understand the Molecular Mechanism of the Interactions of Small Heat Shock Proteins IbpA and IbpB with Lon Protease [C] . Sanchari Bhattacharjee, Rakhi Dasgupta, Angshuman Bagchi International Conference on Information Systems Design and Intelligence Applications . 2015

机译：结构生物信息方法，了解小型热休克蛋白IBPA和IBPB与LON蛋白酶相互作用的分子机制

5. The expression of heat shock protein 70, heat shock protein 90 and heat shock factor 1 within the bovine midcycle corpus luteum after stimulation with temperature in vitro and PGF2alpha in vitro and in vivo. [D] . Kopka, Melissa N. 2006

机译：热激蛋白70，热激蛋白90和热激因子1在体外温度和体外及体内PGF2α刺激后在牛黄体中体中的表达。

6. Defense against Protein Carbonylation by DnaK/DnaJ and Proteases of the Heat Shock Regulon [O] . Åsa Fredriksson, Manuel Ballesteros, Sam Dukan, 2005

机译：DnaK / DnaJ和热休克调节蛋白对蛋白质羰基化的防御作用

7. Defense against Protein Carbonylation by DnaK/DnaJ and Proteases of the Heat Shock Regulon [O] . Fredriksson, Åsa, Ballesteros, Manuel, Dukan, Sam, 2005

机译：DnaK / DnaJ和热休克调节蛋白对蛋白质羰基化的防御作用

8. Detection of the Host Immune Response to Burkholderia mallei Heat-Shock Proteins GroEL and DnaK in a Glanders Patient and Infected Mice [R] . Amemiya, K. , Meyers, J. L. , Deshazer, D. , 2007

机译：在鼻疽患者和感染小鼠中检测对伯克霍尔德氏菌热休克蛋白GroEL和DnaK的宿主免疫应答

1. 热休克蛋白质DnaK的纯化及其二聚体性质研究 [J] . 刘清岱 ,苏家跃 ,田雪利 . 天津科技大学学报 . 2016,第003期

2. 葡萄糖调节蛋白78 kD和热休克蛋白20在左半及右半结肠癌差异表达的初步研究 [J] . 裴海平 ,李小龙 ,朱红 . 中华胃肠外科杂志 . 2013,第001期

3. 基于鸡毒支原体热休克蛋白DnaK的间接ELISA方法的评价及初步应用 [J] . 王莉莉 ,于艳超 ,周长平 . 中国预防兽医学报 . 2018,第009期

4. 热休克蛋白DnaK在大肠杆菌中的克隆与表达 [J] . 陈帅君 ,边靖 . 天津农学院学报 . 2010,第003期

5. 猪肺炎支原体中国分离株热休克蛋白Hsp70(Dnak)的全基因克隆及C末端基因的表达与免疫活性分析 [J] . 李媛 ,张建华 ,王亮 . 中国预防兽医学报 . 2008,第003期

6. 茶黄素对BSA/MDA羰-氨交联反应体系中的蛋白质羰基化及其聚集化抑制作用研究 [C] . 张静 ,蔡淑娴 ,黄建安 . 2016湖南茶业科技创新论坛 . 2016

7. 信号调节蛋白α在树突状细胞活化中的调节作用和射频消融术联合热休克DC疫苗抑制肿瘤复发 [A] . 刘琼 . 2009

1. 弓形虫感染小鼠脑组织差异表达蛋白质、与大脑发育调节蛋白相互作用的蛋白质及其应用 [P] . 中国专利： CN106810602A . 2017-06-09

2. 使用蛋白酶活化的受体鉴别调节蛋白质-蛋白质相互作用的分子 [P] . 中国专利： CN102187225B . 2015.08.05

3. Chaperons DnaK, DnaJ and GrpE from Meiothermus ruber having increased thermal stability and system and process for refolding proteins in vitro [P] . 外国专利： LT5053B . 2003-09-25

机译：Meiothermus ruber的分子伴侣DnaK，DnaJ和GrpE具有更高的热稳定性以及在体外重折叠蛋白质的系统和方法

4. Chaperons DnaK, DnaJ and GrpE from Meiothermus ruber having increased thermal stability and system and process for refolding proteins in vitro [P] . 外国专利： LT2002133A . 2003-07-25

机译：Meiothermus ruber的分子伴侣DnaK，DnaJ和GrpE具有更高的热稳定性以及在体外重折叠蛋白质的系统和方法

5. hhsp60 or an immunogenic fragment thereof, diagnostic test method for a tendency to complement activation induced by heat shock protein, uses of hhsp60 and a member of the hsp60 protein family, other than hhsp60, and of antigen presenting an epitope unique for hhsp60 and that does not have any shared hsp60 epitopes, diagnostic test kit for patients who have a tendency to complement activation induced by heat shock protein, and methods of manufacturing a drug for the treatment of induced complement activation by heat shock protein, and to identify a compound or substance or agent that inhibits hhsp60-induced complement activation [P] . 外国专利： BR0010741A . 2002-02-19

机译：hhsp60或它的免疫原性片段，热休克蛋白诱导的补体激活趋势的诊断测试方法，hhsp60和hhsp60以外的hsp60蛋白质家族成员的用途以及具有hhsp60独特的抗原决定簇的抗原的用途没有任何共享的hsp60表位，倾向于由热激蛋白诱导的补体激活的患者的诊断测试试剂盒以及制造用于治疗由热激蛋白诱导的补体激活并鉴定化合物或物质的药物的方法或抑制hhsp60诱导的补体激活的药物

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Defense against Protein Carbonylation by DnaK/DnaJ and Proteases of the Heat Shock Regulon

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