...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Regions of RNase E Important for 5′-End-Dependent RNA Cleavage and Autoregulated Synthesis
【24h】

Regions of RNase E Important for 5′-End-Dependent RNA Cleavage and Autoregulated Synthesis

机译：RNase E的区域对于5'-端依赖的RNA裂解和自动调节合成很重要

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

RNase E is an important regulatory enzyme that plays a key role in RNA processing and degradation in Escherichia coli. Internal cleavage by this endonuclease is accelerated by the presence of a monophosphate at the RNA 5′ end. Here we show that the preference of E. coli RNase E for 5′-monophosphorylated substrates is an intrinsic property of the catalytically active amino-terminal half of the enzyme and does not require the carboxy-terminal region. This property is shared by the related E. coli ribonuclease CafA (RNase G) and by a cyanobacterial RNase E homolog derived fromSynechocystis, indicating that the 5′-end dependence of RNase E is a general characteristic of members of this ribonuclease family, including those from evolutionarily distant species. Although it is dispensable for 5′-end-dependent RNA cleavage, the carboxy-terminal half of RNase E significantly enhances the ability of this ribonuclease to autoregulate its synthesis in E. coli. Despite similarities in amino acid sequence and substrate specificity, CafA is unable to replace RNase E in sustaining E. colicell growth or in regulating RNase E production, even when overproduced sixfold relative to wild-type RNase E levels.
机译：核糖核酸酶E是一种重要的调节酶，在大肠杆菌的RNA加工和降解中起关键作用。 RNA 5'端单磷酸的存在促进了这种核酸内切酶的内部切割。在这里，我们显示了 E的首选项。用于5′-单磷酸化底物的大肠杆菌RNase E是该酶的催化活性氨基末端一半的固有性质，不需要羧基末端区域。此属性由相关的 E共享。大肠杆菌核糖核酸酶CafA（RNase G）和来自的蓝细菌RNase E同源物，表明RNase E的5'-端依赖性是该核糖核酸酶家族成员的一般特征，包括那些进化距离遥远的物种。尽管对于5'端依赖性RNA切割而言，它是必不可少的，但RNase E的羧基末端一半显着增强了该核糖核酸酶在 E中自动调节其合成的能力。大肠杆菌。尽管氨基酸序列和底物特异性相似，但是CafA在维持 E方面无法替代RNaseE。大肠杆菌细胞的生长或调控RNase E的产生，即使相对于野生型RNase E的水平而言，生产量却增加了六倍。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号