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首页> 外文期刊>Journal of bacteriology >The Mammalian Neuroendocrine Hormone Norepinephrine Supplies Iron for Bacterial Growth in the Presence of Transferrin or Lactoferrin
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The Mammalian Neuroendocrine Hormone Norepinephrine Supplies Iron for Bacterial Growth in the Presence of Transferrin or Lactoferrin

机译：哺乳动物神经内分泌激素去甲肾上腺素在运铁蛋白或乳铁蛋白的存在下为细菌生长提供铁

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Norepinephrine stimulates the growth of a range of bacterial species in nutritionally poor SAPI minimal salts medium containing 30% serum. Addition of size-fractionated serum components to SAPI medium indicated that transferrin was required for norepinephrine stimulation of growth of Escherichia coli. Since bacteriostasis by serum is primarily due to the iron-withholding capacity of transferrin, we considered the possibility that norepinephrine can overcome this effect by supplying transferrin-bound iron for growth. Incubation with concentrations of norepinephrine that stimulated bacterial growth in serum-SAPI medium resulted in loss of bound iron from iron-saturated transferrin, as indicated by the appearance of monoferric and apo- isoforms upon electrophoresis in denaturing gels. Norepinephrine also caused the loss of iron from lactoferrin. The pharmacologically inactive metabolite norepinephrine 3-O-sulfate, by contrast, did not result in iron loss from transferrin or lactoferrin and did not stimulate bacterial growth in serum-SAPI medium. Norepinephrine formed stable complexes with transferrin, lactoferrin, and serum albumin. Norepinephrine-transferrin and norepinephrine-lactoferrin complexes, but not norepinephrine-apotransferrin or norepinephrine-albumin complexes, stimulated bacterial growth in serum-SAPI medium in the absence of additional norepinephrine. Norepinephrine-stimulated growth in medium containing 55Fe complexed with transferrin or lactoferrin resulted in uptake of radioactivity by bacterial cells. Moreover, norepinephrine-stimulated growth in medium containing [3H]norepinephrine indicated concomitant uptake of norepinephrine. In each case, addition of excess iron did not affect growth but significantly reduced levels of radioactivity (55Fe or 3H) associated with bacterial cells. A role for catecholamine-mediated iron supply in the pathophysiology of infectious diseases is proposed.
机译：去甲肾上腺素在营养不良的SAPI基本盐培养基（含30％血清）中刺激多种细菌的生长。在SAPI培养基中添加大小分级的血清成分表明，转铁蛋白是去甲肾上腺素刺激大肠杆菌生长的必需物质。由于血清的抑菌作用主要归因于转铁蛋白的铁截留能力，因此我们考虑了去甲肾上腺素可以通过提供与运铁蛋白结合的铁来生长来克服这种作用的可能性。浓缩去甲肾上腺素刺激血清SAPI培养基中细菌生长的孵育导致了铁饱和转铁蛋白中结合铁的流失，如变性凝胶中电泳时出现的单铁和脱辅基异构体所示。去甲肾上腺素还引起乳铁蛋白中铁的损失。相比之下，无药理活性的去甲肾上腺素3- O -硫酸盐代谢物不会导致转铁蛋白或乳铁蛋白的铁丢失，也不会刺激血清-SAPI培养基中细菌的生长。去甲肾上腺素与运铁蛋白，乳铁蛋白和血清白蛋白形成稳定的复合物。去甲肾上腺素-转铁蛋白和去甲肾上腺素-乳铁蛋白复合物，而不是去甲肾上腺素-载铁蛋白或去甲肾上腺素-白蛋白复合物，在没有其他去甲肾上腺素的情况下刺激血清-SAPI培养基中的细菌生长。去甲肾上腺素刺激含有 55 Fe的铁蛋白与运铁蛋白或乳铁蛋白复合的培养基的生长导致细菌细胞吸收放射性。此外，在含有[ 3 H]去甲肾上腺素的培养基中，去甲肾上腺素刺激的生长指示了去甲肾上腺素的同时摄取。在每种情况下，添加过量的铁都不会影响细菌的生长，但是会显着降低与细菌细胞相关的放射性水平（ 55 Fe或 3 H）。儿茶酚胺介导的铁供应在传染病的病理生理中的作用被提出。

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