A Role for Single-Stranded Exonucleases in the Use of DNA as a Nutrient

Vyacheslav Palchevskiy; Steven E. Finkel

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A Role for Single-Stranded Exonucleases in the Use of DNA as a Nutrient

机译：单链外切核酸在使用DNA作为营养中的作用

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Nutritional competence is the ability of bacterial cells to utilize exogenous double-stranded DNA molecules as a nutrient source. We previously identified several genes in Escherichia coli that are important for this process and proposed a model, based on models of natural competence and transformation in bacteria, where it is assumed that single-stranded DNA (ssDNA) is degraded following entry into the cytoplasm. Since E. coli has several exonucleases, we determined whether they play a role in the long-term survival and the catabolism of DNA as a nutrient. We show here that mutants lacking either ExoI, ExoVII, ExoX, or RecJ are viable during all phases of the bacterial life cycle yet cannot compete with wild-type cells during long-term stationary-phase incubation. We also show that nuclease mutants, alone or in combination, are defective in DNA catabolism, with the exception of the ExoX? single mutant. The ExoX? mutant consumes double-stranded DNA better than wild-type cells, possibly implying the presence of two pathways in E. coli for the processing of ssDNA as it enters the cytoplasm.

机译：营养能力是细菌细胞利用外源双链DNA分子作为营养源的能力。我们以前在大肠杆菌中鉴定了几个对此过程很重要的基因，并基于细菌的自然能力和转化模型提出了一个模型，其中假定单链DNA（ssDNA）为进入细胞质后降解。自 E。大肠杆菌有几种外切核酸酶，我们确定它们是否在长期存活和DNA作为营养物质的分解代谢中起作用。我们在此处显示，缺少ExoI，ExoVII，ExoX或RecJ的突变体在细菌生命周期的所有阶段都是可行的，但在长期固定相孵育期间却无法与野生型细胞竞争。我们还表明，除了ExoX ？单个突变体外，单独或组合的核酸酶突变体在DNA分解代谢方面均存在缺陷。与野生型细胞相比，ExoX ？突变体消耗的双链DNA更好，这可能暗示 E中存在两种途径。大肠杆菌，用于处理进入细胞质的ssDNA。 展开▼

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《Journal of bacteriology》 |2009年第11期|共5页

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Vyacheslav Palchevskiy; Steven E. Finkel;
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中图分类微生物学;

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专利

1. Polynucleotide Kinase and Aprataxin-like Forkhead-associated Protein (PALF) Acts as Both a Single-stranded DNA Endonuclease and a Single-Stranded DNA 3′ Exonuclease and Can Participate in DNA End Joining in a Biochemical System [J] . Sicong Li, Shin-ichiro Kanno, Reiko Watanabe, The Journal of biological chemistry . 2011,第42期

机译：多核苷酸激酶和Aprataxin样叉状蛋白（PALF）用作单链DNA内切核酸酶和单链DNA 3'外切核酸酶，并且可以参与生化系统中的DNA结束

2. 3'-5' Exonuclease of Klenow Fragment: Role of Amino Acid Residues within the Single-Stranded DNA Binding Region in Exonucleolysis and Duplex DNA Melting [J] . Wai-Chung Lam, Elizabeth H.Z.Thompson, Olga Potapova, Biochemistry . 2002,第12期

机译：Klenow片段的3'-5'核酸外切酶：单链DNA结合区域内的氨基酸残基在核酸外切和双链DNA熔解中的作用

3. A novel single-stranded DNA-specific 3'-5' exonuclease, Thermus thermophilus exonuclease I, is involved in several DNA repair pathways. [J] . Shimada A, Masui R, Nakagawa N, Nucleic Acids Research . 2010,第17期

机译：一种新颖的单链DNA特异性3'-5'核酸外切酶，Thermus thermophilus核酸外切酶I，参与了几种DNA修复途径。

4. HDX/MS of the E. coli replicative DNA polymerase identifies stabilizing interactions between the DNA sliding clamp, exonuclease and clamp loader [C] . Rafael Fernandez Leiro, Sarah L. Maslen, Meindert H. Lamers, ASMS Conference on Mass Spectrometry and Allied Topics . 2016

机译：大肠杆菌复制DNA聚合酶的HDX / MS识别DNA滑动夹，外切核酸酶和夹具装载机之间的稳定相互作用

5. Analysis of the interactions between the 5' to 3' exonuclease and the single-stranded DNA-binding protein from bacteriophage T4 and related phages [D] . Boutemy, Laurence S. 2008

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6. A Role for Single-Stranded Exonucleases in the Use of DNA as a Nutrient [O] . Vyacheslav Palchevskiy, Steven E. Finkel 2009

机译：单链外切核酸在使用DNA作为营养中的作用

7. A Role for Single-Stranded Exonucleases in the Use of DNA as a Nutrient▿ [O] . Palchevskiy, Vyacheslav, Finkel, Steven E. 2009

机译：单链外切核酸在使用DNA作为营养素中的作用▿

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机译：原始单链DNA，使用相同DNA制备双链CDNA的方法以及扩增单侧DNA的方法

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A Role for Single-Stranded Exonucleases in the Use of DNA as a Nutrient

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