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首页> 外文期刊>Journal of bacteriology >CcpB, a Novel Transcription Factor Implicated in Catabolite Repression in Bacillus subtilis
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CcpB, a Novel Transcription Factor Implicated in Catabolite Repression in Bacillus subtilis

机译：CcpB，一种新型的转录因子，涉及枯草芽孢杆菌的分解代谢抑制

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Recent work has shown that in Bacillus subtiliscatabolite repression of several operons is mediated by a mechanism dependent on DNA-binding protein CcpA complexed to a seryl-phosphorylated derivative of HPr [HPr(Ser-P)], the small phosphocarrier protein of the phosphoenolpyruvate-sugar phosphotransferase system. In this study, it was found that a transposon insertional mutation resulted in the partial loss of gluconate (gnt) and xylose (xyl) operon catabolite repression by glucose, mannitol, and sucrose. The transposon insertion was localized to a gene, designated ccpB, encoding a protein 30% identical to CcpA, and relief from catabolite repression was shown to be due to the absence of CcpB rather than to the absence of a protein encoded by a downstream gene within the same operon. The relative intensities of CcpA- and CcpB-mediated catabolite repression depended on growth conditions. On solid media, and when cells were grown in liquid media with little agitation, CcpB and CcpA both proved to function in catabolite repression. However, when cells were grown in liquid media with much agitation, CcpA alone mediated catabolite repression. Like CcpA, CcpB appears to exert its catabolite-repressing effect by a mechanism dependent on the presence of HPr(Ser-P).
机译：最近的研究表明，在枯草芽孢杆菌的分解代谢物中，几个操纵子的阻遏作用是由依赖于DNA结合蛋白CcpA的机制介导的，该蛋白质与HPr [HPr（Ser-P）]的丝氨酸磷酸化衍生物复合，磷酸烯醇丙酮酸-糖磷酸转移酶系统的小磷酸载体蛋白。在这项研究中，发现转座子插入突变导致葡萄糖，甘露醇和葡萄糖抑制葡萄糖（ gnt ）和木糖（ xyl ）操纵子分解代谢物的部分丢失。蔗糖。转座子插入被定位在一个名为 ccpB 的基因上，该基因编码的蛋白与CcpA具有30％的相同性，而代谢物阻遏的缓解显示是由于缺少CcpB而不是由于缺少ccpB由同一操纵子内的下游基因编码的蛋白质。 CcpA和CcpB介导的分解代谢物阻遏的相对强度取决于生长条件。在固体培养基上，当细胞在很少搅拌下在液体培养基中生长时，CcpB和CcpA均被证明在分解代谢物抑制中起作用。但是，当细胞在剧烈搅动的液体培养基中生长时，仅CcpA会介导分解代谢物阻遏。与CcpA一样，CcpB似乎通过依赖于HPr（Ser-P）存在的机制发挥其分解代谢物抑制作用。

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