Characterization of the Genes for Two Protocatechuate 3,4-Dioxygenases from the 4-Sulfocatechol-Degrading Bacterium Agrobacterium radiobacter Strain S2

Matthias Contzen; Andreas Stolz

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Characterization of the Genes for Two Protocatechuate 3,4-Dioxygenases from the 4-Sulfocatechol-Degrading Bacterium Agrobacterium radiobacter Strain S2

机译：从4-磺基邻苯二酚降解农杆菌放射细菌菌株S2的两个原儿茶酸3,4-双加氧酶的基因的表征。

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The genes for two different protocatechuate 3,4-dioxygenases (P34Os) were cloned from the 4-sulfocatechol-degrading bacteriumAgrobacterium radiobacter strain S2 (DSMZ 5681). ThepcaH1G1 genes encoded a P34O (P34O-I) which oxidized protocatechuate but not 4-sulfocatechol. These genes were part of a protocatechuate-degradative operon which strongly resembled the isofunctional operon from the protocatechuate-degrading strainAgrobacterium tumefaciens A348 described previously by D. Parke (FEMS Microbiol. Lett. 146:3–12, 1997). The second P34O (P34O-II), encoded by the pcaH2G2 genes, was functionally expressed and shown to convert protocatechuate and 4-sulfocatechol. A comparison of the deduced amino acid sequences of PcaH-I and PcaH-II, and of PcaG-I and PcaG-II, with each other and with the corresponding sequences from the P34Os, from other bacterial genera suggested that the genes for the P34O-II were obtained by strain S2 by lateral gene transfer. The genes encoding the P34O-II were found in a putative operon together with two genes which, according to sequence alignments, encoded transport proteins. Further downstream from this putative operon, two open reading frames which code for a putative regulator protein of the IclR family and a putative 3-carboxymuconate cycloisomerase were identified.

机译：从4-磺基邻苯二酚降解细菌 Agrobacterium radiobacter S2（DSMZ 5681）克隆了两种不同的原儿茶酸3,4-双加氧酶（P34Os）基因。 pcaH1G1 基因编码的P34O（P34O-I）氧化了原儿茶酸而不是4-磺基儿茶酚。这些基因是原儿茶酸降解操纵子的一部分，该操纵子非常类似于原先由D. Parke（FEMS Microbiol。Lett。146：3–12的原发根癌农杆菌A348的同功能操纵子）组成。 1997）。由 pcaH2G2 基因编码的第二个P34O（P34O-II）在功能上得到表达，并显示可以转化原儿茶酸和4-磺基儿茶酚。推导的PcaH-I和PcaH-II氨基酸序列以及PcaG-I和PcaG-II的氨基酸序列彼此之间以及与其他细菌属的P34Os相应序列的比较表明，P34O的基因通过侧向基因转移通过菌株S2获得-II。在推定的操纵子中发现了编码P34O-II的基因，以及两个根据序列比对编码转运蛋白的基因。在该推定操纵子的更下游，鉴定了两个开放阅读框，其编码IclR家族的推定调节蛋白和推定的3-羧基粘康酸酯环异构酶。 展开▼

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《Journal of bacteriology》 |2000年第21期|共7页

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Matthias Contzen; Andreas Stolz;
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1. Molecular Characterization of the GenespcaG and pcaH, Encoding Protocatechuate 3,4-Dioxygenase, Which Are Essential for Vanillin Catabolism inPseudomonas sp. Strain HR199 [J] . J?rg Overhage, Andreas U. Kresse, Horst Priefert, Applied and Environmental Microbiology . 1999,第3期

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2. Positive Selection for Mutations Affecting Bioconversion of Aromatic Compounds in Agrobacterium tumefaciens: Analysis of Spontaneous Mutations in the Protocatechuate 3,4-Dioxygenase Gene [J] . Donna Parke Journal of bacteriology . 2000,第21期

机译：影响根癌土壤杆菌中芳香族化合物生物转化的突变的正选择：原儿茶酸3,4-双加氧酶基因的自发突变分析

3. Characterization of the genes encoding the 3-carboxy-cis,cis-muconate-lactonizing enzymes from the 4-sulfocatechol degradative pathways of Hydrogenophaga intermedia S1 and Agrobacterium radiobacter S2 [J] . Sad Halak, Tamara Basta, Sibylle Bürger, Microbiology . 2006,第11期

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4. Isolation and characterization of 1,2,4-trichlorobenzene degradative bacterium and genetic characterization of its chlorocatechol 1,2-dioxygenase [C] . Li Z M, Gong M Q, Wu J F, Asian Pacific Confederation of Chemical Engineering congress . 2008

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5. Cloning and characterization of protocatechuate 3,4-dioxygenase genes from Bradyrhizobium japonicum USDA110. [D] . Kotagiri, Shailaja. 1994

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7. Characterization of the Genes for Two Protocatechuate 3,4-Dioxygenases from the 4-Sulfocatechol-Degrading Bacterium Agrobacterium radiobacter Strain S2 [O] . Contzen, Matthias, Stolz, Andreas 2000

机译：从4-磺基邻苯二酚降解土壤杆菌土壤杆菌放射细菌菌株S2的两个原儿茶酸3,4-双加氧酶的基因的表征。

8. DNA Sequences of Genes Encoding Acinetobacter calcoaceticus Protocatechuate 3,4-Dioxygenase: Evidence Indicating Shuffling of Genes and of DNA Sequences within Genes during Their Evolutionary Divergence [R] . Hartnett, C., Neidle, E. L., Ngai, K. L., 1990

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Characterization of the Genes for Two Protocatechuate 3,4-Dioxygenases from the 4-Sulfocatechol-Degrading Bacterium Agrobacterium radiobacter Strain S2

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