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首页> 外文期刊>Journal of bacteriology >The CcrM DNA Methyltransferase of Agrobacterium tumefaciens Is Essential, and Its Activity Is Cell Cycle Regulated
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The CcrM DNA Methyltransferase of Agrobacterium tumefaciens Is Essential, and Its Activity Is Cell Cycle Regulated

机译：根癌农杆菌的CcrM DNA甲基转移酶是必不可少的，其活性受细胞周期的调节

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DNA methylation is now recognized as a regulator of multiple bacterial cellular processes. CcrM is a DNA adenine methyltransferase found in the alpha subdivision of the proteobacteria. Like the Dam enzyme, which is found primarily in Escherichia coli and other gamma proteobacteria, it does not appear to be part of a DNA restriction-modification system. The CcrM homolog ofAgrobacterium tumefaciens was found to be essential for viability. Overexpression of CcrM is associated with significant abnormalities of cell morphology and DNA ploidy. Mapping of the transcriptional start site revealed a conserved binding motif for the global response regulator CtrA at the ?35 position; this motif was footprinted by purified Caulobacter crescentus CtrA protein in its phosphorylated state. We have succeeded in isolating synchronized populations of Agrobacterium cells and analyzing their progression through the cell cycle. We demonstrate that DNA replication and cell division can be followed in an orderly manner and that flagellin expression is cyclic, consistent with our observation that motility varies during the cell cycle. Using these synchronized populations, we show that CcrM methylation of the chromosome is restricted to the late S phase of the cell cycle. Thus, within the alpha subdivision, there is a conserved cell cycle dependence and regulatory mechanism controlling ccrMexpression.
机译：DNA甲基化现在被认为是多种细菌细胞过程的调节剂。 CcrM是一种在蛋白质细菌的α细分中发现的DNA腺嘌呤甲基转移酶。像主要在大肠杆菌和其他伽玛变形杆菌中发现的Dam酶一样，它似乎不是DNA限制性修饰系统的一部分。发现根癌农杆菌的CcrM同源物对于生存能力至关重要。 CcrM的过表达与细胞形态和DNA倍性的显着异常有关。转录起始位点的图谱揭示了在35位的全局应答调节子CtrA的保守结合基序。纯化的 crescentbacter crescentus CtrA蛋白处于磷酸化状态时，该基序被覆盖。我们已经成功地分离了农杆菌细胞的同步种群，并分析了它们在整个细胞周期中的进程。我们证明DNA复制和细胞分裂可以有序地进行，并且鞭毛蛋白的表达是循环的，这与我们观察到的运动力在细胞周期中变化是一致的。使用这些同步的种群，我们表明染色体的CcrM甲基化被限制在细胞周期的晚期S期。因此，在alpha细分内，存在保守的细胞周期依赖性和控制 ccrM 表达的调节机制。

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