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首页> 外文期刊>Journal of bacteriology >Vibrio parahaemolyticus scrABC, a Novel Operon Affecting Swarming and Capsular Polysaccharide Regulation
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Vibrio parahaemolyticus scrABC, a Novel Operon Affecting Swarming and Capsular Polysaccharide Regulation

机译：副溶血弧菌scrABC，一种新型的影响成群和荚膜多糖调节的操纵子。

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Swarming is an adaptation of many bacteria to growth on surfaces. A search for genes controlling swarmer cell differentiation of Vibrio parahaemolyticus identified a novel three-gene operon that potentially encodes a pyridoxal-phosphate-dependent enzyme, an extracellular solute-binding protein, and a membrane-bound GGDEF- and EAL-motif sensory protein. The functions of these motifs, which are named after conserved amino acid sequences, are unknown, although the domains are found singly and in combination in a variety of bacterial signaling proteins. Studies with translational fusions supported the predicted localization of the gene products. When the operon was overexpressed, swarmer cell gene transcription was induced in liquid culture. Mutants with defects in any of the three genes exhibited decreased swarming and lateral flagellar (laf) gene expression. Complementation studies confirmed an operon organization and suggested that all three genes participated in laf regulation. The lesions that decreased swarming increased capsular polysaccharide (CPS) production, and overexpression of the operon inhibited transcription of the CPS gene cpsA. Thus, the scrABC locus appears to inversely regulate two gene systems that are pertinent to colonization of surface swarming and CPS.
机译：集群是许多细菌对表面生长的适应。对控制溶血性弧菌的成群细胞分化的基因进行搜索后，发现了一个新型的三基因操纵子，该操纵子可能编码吡pyr醛磷酸盐依赖性酶，胞外溶质结合蛋白和膜结合型GGDEF-。和EAL基序感觉蛋白。这些基序的功能以保守的氨基酸序列命名，尽管在多个细菌信号蛋白中单独或结合发现了这些结构域，但这些功能未知。翻译融合的研究支持了基因产物的预期定位。当操纵子过表达时，在液体培养中诱导了成群的细胞基因转录。这三个基因中任何一个都具有缺陷的突变体表现出群聚和侧鞭毛（ laf ）基因表达降低。补充研究证实了操纵子的组织，并建议所有三个基因都参与了 laf 调节。蜂群减少的病灶增加了荚膜多糖（CPS）的产生，操纵子的过表达抑制了CPS基因 cpsA 的转录。因此， scrABC 基因座似乎反向调节了两个与表面虫群和CPS定殖有关的基因系统。

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