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首页> 外文期刊>Journal of bacteriology >Peculiar Properties of DsbA in Its Export across the Escherichia coli Cytoplasmic Membrane
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Peculiar Properties of DsbA in Its Export across the Escherichia coli Cytoplasmic Membrane

机译：DsbA在其跨大肠杆菌细胞质膜的出口中的特殊特性

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摘要

Export of DsbA, a protein disulfide bond-introducing enzyme, across the Escherichia coli cytoplasmic membrane was studied with special reference to the effects of various mutations affecting translocation factors. It was noted that both the internalized precursor retaining the signal peptide and the periplasmic mature product fold rapidly into a protease-resistant structure and they exhibited anomalies in sodium dodecyl sulfate-polyacrylamide gel electrophoresis in that the former migrated faster than the latter. The precursor, once accumulated, was not exported posttranslationally. DsbA export depended on the SecY translocon, the SecA ATPase, and Ffh (signal recognition particle), but not on SecB. SecY mutations, such as secY39 and secY205, that severely impair translocation of a number of secretory substrates by interfering with SecA actions only insignificantly impaired the DsbA export. In contrast, secY125, affecting a periplasmic domain and impairing a late step of translocation, exerted strong export inhibition of both classes of proteins. These results suggest that DsbA uses not only the signal recognition particle targeting pathway but also a special route of translocation through the translocon, which is hence suggested to actively discriminate preproteins.
机译：研究了DsbA（一种蛋白质二硫键导入酶）在整个大肠杆菌细胞质膜上的出口，并特别参考了各种突变影响易位因子的作用。注意到保留信号肽的内化前体和周质成熟产物均迅速折叠成蛋白酶抗性结构，并且它们在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中表现出异常，因为前者比后者迁移更快。前体一旦积累，就不会翻译后导出。 DsbA出口取决于SecY转运子，SecA ATPase和Ffh（信号识别颗粒），而不取决于SecB。 SecY突变（例如 secY39 和 secY205 ）通过干扰SecA行为而严重损害了许多分泌性底物的转运，而对DsbA的输出影响却很小。相反， secY125 会影响周质结构域并损害转位的后期步骤，对这两类蛋白质都具有强大的输出抑制作用。这些结果表明，DsbA不仅使用信号识别颗粒靶向途径，而且还使用通过转位子的特殊易位途径，因此建议主动区分前蛋白。

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