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首页> 外文期刊>Journal of bacteriology >Hfq Modulates the σE-Mediated Envelope Stress Response and the σ32-Mediated Cytoplasmic Stress Response in Escherichia coli
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Hfq Modulates the σE-Mediated Envelope Stress Response and the σ32-Mediated Cytoplasmic Stress Response in Escherichia coli

机译：Hfq调节大肠杆菌中σE介导的包膜应力响应和σ32介导的细胞质应力响应

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摘要

Hfq, a chaperone for small noncoding RNAs, regulates many processes in Escherichia coli, including the σS-mediated general stress response. Here we used microarray analysis to identify the changes in gene expression resulting from lack of Hfq. We identify several potential new targets for Hfq regulation, including genes encoding outer membrane proteins, enzymes, factors, and transporters. Many of these genes are involved in amino acid uptake and biosynthesis, sugar uptake and metabolism, and cell energetics. In addition, we find altered regulation of the σE- and σ32-mediated stress responses, which we analyze further. We show that cells lacking Hfq induce the σE-mediated envelope stress response and are defective in σE-mediated repression of outer membrane proteins. We also show that the σ32-mediated cytoplasmic stress response is repressed in cells lacking Hfq due to increased expression of DnaK. Furthermore, we show that cells lacking Hfq are defective in the “long-term adaptation” of σ32 to chronic chaperone overexpression. Together, our results indicate that Hfq may play a general role in stress response regulation in E. coli.
机译：Hfq是一种针对小型非编码RNA的分子伴侣，可调节大肠杆菌中的许多过程，包括σ S 介导的一般应激反应。在这里，我们使用微阵列分析来确定由于缺乏Hfq而导致的基因表达变化。我们确定了Hfq调节的几个潜在的新目标，包括编码外膜蛋白，酶，因子和转运蛋白的基因。这些基因中的许多都涉及氨基酸的摄取和生物合成，糖的摄取和代谢以及细胞能量学。此外，我们发现σ E -和σ 32 介导的应力反应的调控发生了变化，我们对此进行了进一步分析。我们发现缺乏Hfq的细胞会诱导σ E 介导的包膜应激反应，并且在σ E 介导的外膜蛋白阻遏中存在缺陷。我们还显示，由于DnaK的表达增加，在缺乏Hfq的细胞中σ 32 介导的细胞质应激反应受到抑制。此外，我们表明缺乏Hfq的细胞在σ 32 的“长期适应”中对慢性伴侣伴侣的过度表达存在缺陷。总之，我们的结果表明，Hfq可能在 E的应激反应调节中起一般作用。大肠杆菌。

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