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首页> 外文期刊>Journal of bacteriology >A Serratia marcescens OxyR Homolog Mediates Surface Attachment and Biofilm Formation
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A Serratia marcescens OxyR Homolog Mediates Surface Attachment and Biofilm Formation

机译：粘质沙雷氏菌OxyR同源物介导表面附着和生物膜形成。

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OxyR is a conserved bacterial transcription factor with a regulatory role in oxidative stress response. From a genetic screen for genes that modulate biofilm formation in the opportunistic pathogen Serratia marcescens, mutations in an oxyR homolog and predicted fimbria structural genes were identified. S. marcescens oxyR mutants were severely impaired in biofilm formation, in contrast to the hyperbiofilm phenotype exhibited by oxyR mutants of Escherichia coli and Burkholderia pseudomallei. Further analysis revealed that OxyR plays a role in the primary attachment of cells to a surface. Similar to what is observed in other bacterial species, S. marcescens OxyR is required for oxidative stress resistance. Mutations in oxyR and type I fimbrial genes resulted in severe defects in fimbria-associated phenotypes, revealing roles in cell-cell and cell-biotic surface interactions. Transmission electron microscopy revealed the absence of fimbria-like surface structures on an OxyR-deficient strain and an enhanced fimbrial phenotype in strains bearing oxyR on a multicopy plasmid. The hyperfimbriated phenotype conferred by the multicopy oxyR plasmid was absent in a type I fimbrial mutant background. Real-time reverse transcriptase PCR indicated an absence of transcripts from a fimbrial operon in an oxyR mutant that were present in the wild type and a complemented oxyR mutant strain. Lastly, chromosomal Plac-mediated expression of fimABCD was sufficient to restore wild-type levels of yeast agglutination and biofilm formation to an oxyR mutant. Together, these data support a model in which OxyR contributes to early stages of S. marcescens biofilm formation by influencing fimbrial gene expression.
机译：OxyR是一种保守的细菌转录因子，在氧化应激反应中具有调节作用。从基因筛选的基因调控机会性病原体粘质沙雷氏菌中的生物膜形成，确定了 oxyR 同系物的突变和预测的菌毛结构基因。 S。 marcescens oxyR 突变体严重损害了生物膜的形成，与大肠杆菌和 Burkholderia pseudomallei oxyR 突变体表现出的高生物膜表型相反>。进一步的分析表明，OxyR在细胞与表面的初步附着中起作用。与在其他细菌中观察到的类似。 marcescens OxyR是抗氧化应激的必需物质。 oxyR 和I型纤维基因的突变导致与菌毛相关的表型出现严重缺陷，揭示了细胞与细胞和生物表面相互作用中的作用。透射电子显微镜显示在多拷贝质粒上带有 oxyR 的菌株中，在OxyR缺陷型菌株上不存在菌毛状表面结构，而纤维表型增强。在I型纤维突变体背景下，没有多拷贝 oxyR 质粒赋予的超纤维化表型。实时逆转录酶PCR表示野生型和互补型 oxyR 突变株中存在 oxyR 突变体中来自纤维操纵子的转录本。最后，染色体 P lac 介导的 fimABCD 表达足以将酵母菌的野生型水平和生物膜形成恢复到 oxyR 突变体。这些数据共同支持了OxyR有助于 S早期阶段的模型。影响纤维基因表达的粘质囊菌生物膜形成

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