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首页> 外文期刊>Journal of bacteriology >Bioinformatic and Biochemical Evidence for the Identification of the Type III Secretion System Needle Protein of Chlamydia trachomatis
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Bioinformatic and Biochemical Evidence for the Identification of the Type III Secretion System Needle Protein of Chlamydia trachomatis

机译：沙眼衣原体III型分泌系统针蛋白鉴定的生物信息学和生化证据

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Chlamydia spp. express a functional type III secretion system (T3SS) necessary for pathogenesis and intracellular growth. However, certain essential components of the secretion apparatus have diverged to such a degree as to preclude their identification by standard homology searches of primary protein sequences. One example is the needle subunit protein. Electron micrographs indicate that chlamydiae possess needle filaments, and yet database searches fail to identify a SctF homologue. We used a bioinformatics approach to identify a likely needle subunit protein for Chlamydia. Experimental evidence indicates that this protein, designated CdsF, has properties consistent with it being the major needle subunit protein. CdsF is concentrated in the outer membrane of elementary bodies and is surface exposed as a component of an extracellular needle-like projection. During infection CdsF is detectible by indirect immunofluorescence in the inclusion membrane with a punctuate distribution adjacent to membrane-associated reticulate bodies. Biochemical cross-linking studies revealed that, like other SctF proteins, CdsF is able to polymerize into multisubunit complexes. Furthermore, we identified two chaperones for CdsF, termed CdsE and CdsG, which have many characteristics of the Pseudomonas spp. needle chaperones PscE and PscG, respectively. In aggregate, our data are consistent with CdsF representing at least one component of the extended Chlamydia T3SS injectisome. The identification of this secretion system component is essential for studies involving ectopic reconstitution of the Chlamydia T3SS. Moreover, we anticipate that CdsF could serve as an efficacious target for anti-Chlamydia neutralizing antibodies.
机译：衣原体 spp。表达功能性III型分泌系统（T3SS）的发病机制和细胞内生长所必需。然而，该分泌装置的某些基本组成部分已发生分歧，以致于不能通过对一级蛋白质序列的标准同源性搜索来鉴定它们。一个例子是针状亚基蛋白。电子显微照片表明衣原体具有针状细丝，但数据库搜索未能鉴定出SctF同源物。我们使用生物信息学方法确定了衣原体的可能的针头亚基蛋白。实验证据表明，这种称为CdsF的蛋白质具有与作为主要的针状亚基蛋白质一致的特性。 CdsF集中在基本体的外膜中，并作为细胞外针状突起的一部分暴露在表面。在感染过程中，可通过包涵膜中的间接免疫荧光检测CdsF，其点状分布邻近与膜相关的网状体。生化交联研究表明，与其他SctF蛋白一样，CdsF能够聚合成多亚基复合物。此外，我们确定了CdsF的两个伴侣，分别称为CdsE和CdsG，它们具有 Pseudomonas spp的许多特征。针伴侣蛋白PscE和PscG。总体而言，我们的数据与CdsF一致，后者代表扩展的衣原体 T3SS注射体的至少一种成分。该分泌系统成分的鉴定对于涉及衣原体 T3SS异位重建的研究至关重要。此外，我们预期CdsF可以作为抗<衣原体中和抗体的有效靶标。

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