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首页> 外文期刊>Journal of bacteriology >A Common Virulence Plasmid in Biotype 2 Vibrio vulnificus and Its Dissemination Aided by a Conjugal Plasmid
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A Common Virulence Plasmid in Biotype 2 Vibrio vulnificus and Its Dissemination Aided by a Conjugal Plasmid

机译：生物型2型弧菌弧菌中的一种常见毒力质粒及其配偶质粒的辅助传播

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摘要

Strains of Vibrio vulnificus, a marine bacterial species pathogenic for humans and eels, are divided into three biotypes, and those virulent for eels are classified as biotype 2. All biotype 2 strains possess one or more plasmids, which have been shown to harbor the biotype 2-specific DNA sequences. In this study we determined the DNA sequences of three biotype 2 plasmids: pR99 (68.4 kbp) in strain CECT4999 and pC4602-1 (56.6 kb) and pC4602-2 (66.9 kb) in strain CECT4602. Plasmid pC4602-2 showed 92% sequence identity with pR99. Curing of pR99 from strain CECT4999 resulted in loss of resistance to eel serum and virulence for eels but had no effect on the virulence for mice, an animal model, and resistance to human serum. Plasmids pC4602-2 and pR99 could be transferred to the plasmid-cured strain by conjugation in the presence of pC4602-1, which was self-transmissible, and acquisition of pC4602-2 restored the virulence of the cured strain for eels. Therefore, both pR99 and pC4602-2 were virulence plasmids for eels but not mice. A gene in pR99, which encoded a novel protein and had an equivalent in pC4602-2, was further shown to be essential, but not sufficient, for the resistance to eel serum and virulence for eels. There was evidence showing that pC4602-2 may form a cointegrate with pC4602-1. An investigation of six other biotype 2 strains for the presence of various plasmid markers revealed that they all harbored the virulence plasmid and four of them possessed the conjugal plasmid in addition.
机译：对人类和鳗鱼致病的海洋弧菌 Vibrio vulnificus 菌株分为三种生物型，对鳗鱼有毒力的菌株被分类为生物型2。所有生物型2菌株均具有一个或多个质粒，其中已显示出具有生物型2特异性DNA序列。在这项研究中，我们确定了三种2型生物质粒的DNA序列：菌株CECT4999中的pR99（68.4 kbp）和菌株CECT4602中的pC4602-1（56.6 kb）和pC4602-2（66.9 kb）。质粒pC4602-2与pR99显示92％的序列同一性。来自菌株CECT4999的pR99的固化导致对鳗鱼血清的抗性和鳗鱼的毒性丧失，但是对小鼠，动物模型的毒力和对人血清的抗性没有影响。质粒pC4602-2和pR99可以在pC4602-1存在的情况下通过结合转移到质粒固化的菌株中，而pC4602-1是可自我传播的，pC4602-2的获得恢复了该固化菌株对鳗鱼的毒力。因此，pR99和pC4602-2都是鳗鱼的毒力质粒，而不是小鼠的毒力质粒。进一步显示，pR99中的一个基因编码一种新蛋白质，并在pC4602-2中具有相同的基因，对于抗鳗鱼血清和鳗鱼的毒力而言，这是必不可少的，但不足。有证据表明，pC4602-2可能与pC4602-1形成共积分。对另外六种生物2型菌株进行的各种质粒标记物研究表明，它们都带有毒力质粒，其中四株还具有接合质粒。

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