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首页> 外文期刊>Journal of bacteriology >ldpA Encodes an Iron-Sulfur Protein Involved in Light-Dependent Modulation of the Circadian Period in the Cyanobacterium Synechococcus elongatus PCC 7942
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ldpA Encodes an Iron-Sulfur Protein Involved in Light-Dependent Modulation of the Circadian Period in the Cyanobacterium Synechococcus elongatus PCC 7942

机译：ldpA编码一种铁硫蛋白，参与长蓝细菌蓝藻PCC 7942昼夜节律的光依赖性调节

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We generated random transposon insertion mutants to identify genes involved in light input pathways to the circadian clock of the cyanobacterium Synechococcus elongatus PCC 7942. Two mutants, AMC408-M1 and AMC408-M2, were isolated that responded to a 5-h dark pulse differently from the wild-type strain. The two mutants carried independent transposon insertions in an open reading frame here named ldpA (for light-dependent period). Although the mutants were isolated by a phase shift screening protocol, the actual defect is a conditional alteration in the circadian period. The mutants retain the wild-type ability to phase shift the circadian gene expression (bioluminescent reporter) rhythm if the timing of administration of the dark pulse is corrected for a 1-h shortening of the circadian period in the mutant. Further analysis indicated that the conditional short-period mutant phenotype results from insensitivity to light gradients that normally modulate the circadian period in S. elongatus, lengthening the period at low light intensities. The ldpA gene encodes a polypeptide that predicts a 7Fe-8S cluster-binding motif expected to be involved in redox reactions. We suggest that the LdpA protein modulates the circadian clock as an indirect function of light intensity by sensing changes in cellular physiology.
机译：我们生成了随机转座子插入突变体，以识别参与蓝细菌 Synechococcus elongatus PCC 7942的生物钟的光输入途径的基因。两个突变体，AMC408-M1和AMC408-M2分离出对5-h暗脉冲的响应不同于野生型菌株。这两个突变体在一个开放阅读框（此处称为 ldpA ）中进行了独立的转座子插入（针对光依赖期）。尽管通过相移筛选方案分离了突变体，但实际缺陷是昼夜节律期间的条件改变。如果将暗脉冲的施药时间校正为突变体中昼夜节律的1-h缩短，则突变体将保留野生型相控昼夜节律基因表达（生物发光报告基因）节奏的能力。进一步的分析表明，条件短周期突变体表型是由于对通常调节 S 中昼夜节律周期的光梯度不敏感所致。 elongatus ，延长了弱光下的时间。 ldpA 基因编码预测7Fe-8S簇结合基序的多肽，该基序有望参与氧化还原反应。我们建议LdpA蛋白通过检测细胞生理学的变化，将昼夜节律作为光强度的间接函数进行调节。

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