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首页> 外文期刊>Journal of bacteriology >Crystal Structure of the “PhoU-Like” Phosphate Uptake Regulator from Aquifex aeolicus
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Crystal Structure of the “PhoU-Like” Phosphate Uptake Regulator from Aquifex aeolicus

机译：Aquifex aeolicus的“ PhoU样”磷酸盐吸收调节剂的晶体结构

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The phoU gene of Aquifex aeolicus encodes a protein called PHOU_AQUAE with sequence similarity to the PhoU protein of Escherichia coli. Despite the fact that there is a large number of family members (more than 300) attributed to almost all known bacteria and despite PHOU_AQUAE's association with the regulation of genes for phosphate metabolism, the nature of its regulatory function is not well understood. Nearly one-half of these PhoU-like proteins, including both PHOU_AQUAE and the one from E. coli, form a subfamily with an apparent dimer structure of two PhoU domains on the basis of their amino acid sequence. The crystal structure of PHOU_AQUAE (a 221-amino-acid protein) reveals two similar coiled-coil PhoU domains, each forming a three-helix bundle. The structures of PHOU_AQUAE proteins from both a soluble fraction and refolded inclusion bodies (at resolutions of 2.8 and 3.2?, respectively) showed no significant differences. The folds of the PhoU domain and Bag domains (for a class of cofactors of the eukaryotic chaperone Hsp70 family) are similar. Accordingly, we propose that gene regulation by PhoU may occur by association of PHOU_AQUAE with the ATPase domain of the histidine kinase PhoR, promoting release of its substrate PhoB. Other proteins that share the PhoU domain fold include the coiled-coil domains of the STAT protein, the ribosome-recycling factor, and structural proteins like spectrin.
机译：尽管水曲柳中有一个大肠杆菌，但 Aquifex aeolicus 的 phoU 基因编码的一种蛋白叫做PHOU_AQUAE，其序列与大肠杆菌的PhoU蛋白具有序列相似性。大量的家庭成员（超过300个）归因于几乎所有已知细菌，尽管PHOU_AQUAE与磷酸盐代谢基因的调节有关，但对其调节功能的性质尚不甚了解。这些PhoU样蛋白中将近一半，包括PHOU_AQUAE和 E中的一种。大肠杆菌形成一个亚家族，根据其氨基酸序列，两个PhoU结构域具有明显的二聚体结构。 PHOU_AQUAE（一种221个氨基酸的蛋白质）的晶体结构显示出两个相似的卷曲螺旋PhoU域，每个域都形成一个三螺旋束。来自可溶性级分和重折叠的包涵体的PHOU_AQUAE蛋白的结构（分别为2.8和3.2？的分辨率）没有显着差异。 PhoU域和Bag域的折叠（对于真核伴侣Hsp70家族的一类辅助因子）是相似的。因此，我们建议通过PHOU_AQUAE与组氨酸激酶PhoR的ATPase域的结合来发生PhoU的基因调控，从而促进其底物PhoB的释放。共享PhoU结构域折叠的其他蛋白包括STAT蛋白的卷曲螺旋结构域，核糖体循环因子和结构蛋白（如血影蛋白）。

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