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首页> 外文期刊>Journal of bacteriology >LytF, a Novel Competence-Regulated Murein Hydrolase in the Genus Streptococcus
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LytF, a Novel Competence-Regulated Murein Hydrolase in the Genus Streptococcus

机译：LytF，链球菌属中一种新的能力调节的Murein水解酶

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Streptococcus pneumoniae and probably most other members of the genus Streptococcus are competent for natural genetic transformation. During the competent state, S. pneumoniae produces a murein hydrolase, CbpD, that kills and lyses noncompetent pneumococci and closely related species. Previous studies have shown that CbpD is essential for efficient transfer of genomic DNA from noncompetent to competent cells in vitro. Consequently, it has been proposed that CbpD together with the cognate immunity protein ComM constitutes a DNA acquisition mechanism that enables competent pneumococci to capture homologous DNA from closely related streptococci sharing the same habitat. Although genes encoding CbpD homologs or CbpD-related proteins are present in many different streptococcal species, the genomes of a number of streptococci do not encode CbpD-type proteins. In the present study we show that the genomes of nearly all species lacking CbpD encode an unrelated competence-regulated murein hydrolase termed LytF. Using Streptococcus gordonii as a model system, we obtained evidence indicating that LytF is a functional analogue of CbpD. In sum, our results show that a murein hydrolase gene is part of the competence regulon of most or all streptococcal species, demonstrating that these muralytic enzymes constitute an essential part of the streptococcal natural transformation system.
机译：肺炎链球菌和链球菌属的大多数其他成员都可以胜任自然遗传转化。在胜任状态期间，肺炎链球菌产生了鼠莫林水解酶CbpD，该酶杀死并溶解了非胜任的肺炎球菌和密切相关的物种。以前的研究表明，CbpD对于将基因组DNA从非感受态细胞有效地转移到体外 至关重要。因此，已经提出CbpD与同源免疫蛋白ComM一起构成了一种DNA获取机制，该机制使能胜任的肺炎链球菌能够从共享同一栖息地的密切相关的链球菌中捕获同源DNA。尽管在许多不同的链球菌物种中都存在编码CbpD同源物或CbpD相关蛋白的基因，但是许多链球菌的基因组都不编码CbpD型蛋白。在本研究中，我们表明几乎所有缺乏CbpD的物种的基因组都编码一种称为LytF的不相关的能力调节型Murein水解酶。使用戈登链球菌作为模型系统，我们获得了表明LytF是CbpD的功能类似物的证据。总而言之，我们的研究结果表明，鼠莫林水解酶基因是大多数或所有链球菌物种能力调节的一部分，表明这些易分解酶构成了链球菌自然转化系统的重要组成部分。

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