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首页> 外文期刊>Journal of bacteriology >Proteomic Analysis of the Spore Coats of Bacillus subtilis and Bacillus anthracis
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Proteomic Analysis of the Spore Coats of Bacillus subtilis and Bacillus anthracis

机译：枯草芽孢杆菌和炭疽芽孢杆菌孢子衣壳的蛋白质组学分析

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The outermost proteinaceous layer of bacterial spores, called the coat, is critical for spore survival, germination, and, for pathogenic spores, disease. To identify novel spore coat proteins, we have carried out a preliminary proteomic analysis of Bacillus subtilis and Bacillus anthracis spores, using a combination of standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis separation and improved two-dimensional electrophoretic separations, followed by matrix-assisted laser desorption ionization-time of flight and/or dual mass spectrometry. We identified 38 B. subtilis spore proteins, 12 of which are known coat proteins. We propose that, of the novel proteins, YtaA, YvdP, and YnzH are bona fide coat proteins, and we have renamed them CotI, CotQ, and CotU, respectively. In addition, we initiated a study of coat proteins in B. anthracis and identified 11 spore proteins, 6 of which are candidate coat or exosporium proteins. We also queried the unfinished B. anthracis genome for potential coat proteins. Our analysis suggests that the B. subtilis and B. anthracis coats have roughly similar numbers of proteins and that a core group of coat protein species is shared between these organisms, including the major morphogenetic proteins. Nonetheless, a significant number of coat proteins are probably unique to each species. These results should accelerate efforts to develop B. anthracis detection methods and understand the ecological role of the coat.
机译：细菌孢子的最外层蛋白质层（称为外壳）对于孢子的存活，发芽以及致病性孢子的疾病至关重要。为了鉴定新的孢子外壳蛋白，我们使用标准十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离技术对枯草芽孢杆菌和炭疽芽孢杆菌孢子进行了蛋白质组学初步分析改进的二维电泳分离，然后进行基质辅助的激光解吸电离飞行时间和/或双重质谱。我们确定了38个 B 。枯草芽孢蛋白，其中12种是已知的外壳蛋白。我们提出，在新型蛋白质中，YtaA，YvdP和YnzH是真正的外壳蛋白，并且我们分别将其重命名为CotI，CotQ和CotU。此外，我们开始研究 B 中的外壳蛋白。 炭疽并鉴定出11种孢子蛋白，其中6种是候选外壳蛋白或外孢子蛋白。我们还查询了未完成的 B 。可能的外壳蛋白的炭疽基因组。我们的分析表明 B 。 枯草和 B 。 炭疽的外皮中蛋白质的数量大致相似，并且这些生物体（包括主要的形态发生蛋白）之间共享一个核心的外皮蛋白质种类。但是，每种物种可能都存在大量的外壳蛋白。这些结果将加快开发 B 的努力。 炭疽检测方法，了解大衣的生态作用。

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