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首页> 外文期刊>Journal of applied microbiology >Mechanism of killing of spores of Bacillus anthracis in a high-temperature gas environment, and analysis of DNA damage generated by various decontamination treatments of spores of Bacillus anthracis, Bacillus subtilis and Bacillus thuringiensis.
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Mechanism of killing of spores of Bacillus anthracis in a high-temperature gas environment, and analysis of DNA damage generated by various decontamination treatments of spores of Bacillus anthracis, Bacillus subtilis and Bacillus thuringiensis.

机译：在高温气体环境中杀死炭疽芽孢杆菌孢子的机理，以及炭疽芽孢杆菌，枯草芽孢杆菌和苏云金芽孢杆菌的各种去污处理产生的DNA损伤的分析。

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Aims: To determine how hydrated Bacillus anthracis spores are killed in a high-temperature gas environment (HTGE), and how spores of several Bacillus species including B. anthracis are killed by UV radiation, dry heat, wet heat and desiccation. Methods and Results: Hydrated B. anthracis spores were HTGE treated at c. 220 degrees C for 50 ms, and the treated spores were tested for germination, mutagenesis, rupture and loss of dipicolinic acid. Spores of this and other Bacillus species were also examined for mutagenesis by UV, wet and dry heat and desiccation. There was no rupture of HTGE-treated B. anthracis spores killed 90-99.9%, no mutagenesis, and release of DPA and loss of germination were much slower than spore killing. However, killing of spores of B. anthracis, Bacillus thuringiensis and Bacillus subtilis by UV radiation or dry heat, but not wet heat in water or ethanol, was accompanied by mutagenesis. Conclusions: It appears likely that HTGE treatment kills B. anthracis spores by damage to spore core proteins. In addition, various killing regimens inactivate spores of a number of Bacillus species by the same mechanisms. Significance and Impact of the Study: This work indicates how hydrated spores treated in a HTGE such as might be used to destroy biological warfare agent stocks are killed. The work also indicates that mechanisms whereby different agents kill spores are similar with spores of different Bacillus species.
机译：目的：确定在高温气体环境（HTGE）中如何杀死水合炭疽芽孢杆菌的孢子，以及如何通过紫外线辐射，干热，湿热和干燥作用杀死包括炭疽芽孢杆菌在内的几种芽孢杆菌种的孢子。方法和结果：水合炭疽杆菌的孢子经HTGE处理。在220摄氏度下持续50毫秒，并测试了处理过的孢子的发芽，诱变，破裂和二吡啶甲酸的损失。还通过紫外线，湿热和干热以及干燥来检查该芽孢杆菌和其他芽孢杆菌的孢子是否诱变。经HTGE处理的炭疽芽孢杆菌没有破裂，杀死了90-99.9％，没有诱变，DPA的释放和发芽的损失比杀死孢子要慢得多。然而，诱变伴随着紫外线辐射或干热而不是湿热在水或乙醇中杀死炭疽芽孢杆菌，苏云金芽孢杆菌和枯草芽孢杆菌的孢子。结论：HTGE处理似乎通过破坏孢子核心蛋白杀死了炭疽杆菌的孢子。另外，各种杀灭方案通过相同的机理使许多芽孢杆菌属的孢子失活。研究的意义和影响：这项工作表明了在HTGE中处理过的水合孢子（例如可用于破坏生物战剂库）的杀灭方式。这项工作还表明，不同药剂杀死孢子的机制与不同芽孢杆菌种的孢子相似。

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