...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Identification, Source, and Metabolism of N-Ethylglutamate in Escherichia coli
【24h】

Identification, Source, and Metabolism of N-Ethylglutamate in Escherichia coli

机译：大肠杆菌中N-乙基谷氨酸的鉴定，来源和代谢

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

N-Ethylglutamate (NEG) was detected in Escherichia coli BL21 cells grown on LB broth, and it was found to occur at a concentration of ～4 mM in these cells under these conditions. The same cells grown on M9 glucose medium contained no detectable amount of NEG. Analysis of the LB broth showed the presence of NEG, a compound never before reported as a natural product. Isotope dilution analysis showed that it occurred at a concentration of 160 μM in LB broth. Analyses of yeast extract and tryptone, the organic components of LB broth, both showed the presence NEG. It was demonstrated that NEG can be generated during the autolysis of the yeast used in the preparation of the yeast extract. Growth of these E. coli cells in LB broth prepared in deuterated water showed no incorporation of deuterium into NEG, demonstrating that E. coli cells did not generate the NEG. Cell growth rates were not affected by the addition of 5 mM NEG to either LB or M9 glucose medium. l-[ethyl-2H4]NEG was found to be readily incorporated into the cells and metabolized by the cells. From these results, it was concluded that all of the NEG present in the cells was taken up from the medium. NEG could serve as the sole nitrogen source for E. coli when grown on M9 glucose medium in the presence of glucose but could not serve as the sole carbon source on M9 medium in the absence of glucose.
机译：在LB肉汤中生长的大肠杆菌 BL21细胞中检测到 N -乙基谷氨酸（NEG），发现在这些细胞下，这些细胞中的浓度为〜4 mM。条件。在M9葡萄糖培养基上生长的相同细胞不含可检测量的NEG。 LB肉汤的分析显示NEG的存在，NEG是从未报道过的天然产物。同位素稀释分析表明，它在LB肉汤中的浓度为160μM。 LB肉汤的有机成分酵母提取物和胰蛋白try的分析均显示存在NEG。已经证明，在酵母提取物的制备中使用的酵母的自溶过程中可以产生NEG。这些 E的增长。用氘水制备的LB肉汤中的大肠杆菌细胞没有显示出氘掺入NEG中，证明了 E。大肠杆菌细胞不产生NEG。向LB或M9葡萄糖培养基中添加5 mM NEG不会影响细胞生长速度。发现1-[乙基- 2 H 4 ] NEG易于掺入细胞并被细胞代谢。从这些结果可以得出结论，存在于细胞中的所有NEG都是从培养基中吸收的。 NEG可以作为 E的唯一氮源。大肠杆菌在存在葡萄糖的M9葡萄糖培养基上生长，但是在缺乏葡萄糖的情况下不能作为M9培养基上的唯一碳源。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号