...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >MdoR Is a Novel Positive Transcriptional Regulator for the Oxidation of Methanol in Mycobacterium sp. Strain JC1
【24h】

MdoR Is a Novel Positive Transcriptional Regulator for the Oxidation of Methanol in Mycobacterium sp. Strain JC1

机译：MdoR是一种新颖的正转录调节剂，用于氧化分枝杆菌中的甲醇。菌株JC1

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mycobacterium sp. strain JC1 is able to grow on methanol as a sole source of carbon and energy using methanol:N,N′-dimethyl-4-nitrosoaniline oxidoreductase (MDO) as a key enzyme for methanol oxidation. The second open reading frame (mdoR) upstream of, and running divergently from, the mdo gene was identified as a gene for a TetR family transcriptional regulator. The N-terminal region of MdoR contained a helix-turn-helix DNA-binding motif. An electrophoretic mobility shift assay (EMSA) indicated that MdoR could bind to a mdo promoter region containing an inverted repeat. The mdoR deletion mutant did not grow on methanol, but growth on methanol was restored by a plasmid containing an intact mdoR gene. In DNase I footprinting and EMSA experiments, MdoR bound to two inverted repeats in the putative mdoR promoter region. Reverse transcription-PCR indicated that the mdoR gene was transcribed only in cells growing on methanol, whereas β-galactosidase assays showed that the mdoR promoter was activated in the presence of methanol. These results indicate that MdoR serves as a transcriptional activator for the expression of mdo and its own gene. Also, MdoR is the first discovered member of the TetR family of transcriptional regulators to be involved in the regulation of the methanol oxidation, as well as to function as a positive autoregulator.
机译：分枝杆菌使用甲醇： N ， N'-二甲基-4-亚硝基苯胺氧化还原酶（MDO）作为关键，菌株JC1能够在甲醇上作为碳和能量的唯一来源生长甲醇氧化酶。第二个开放阅读框（ mdoR ）位于 mdo 基因的上游并从其发散而来，被确定为TetR家族转录调节子的基因。 MdoR的N端区域包含一个螺旋-转-螺旋-DNA结合基序。电泳迁移率迁移分析（EMSA）表明，MdoR可以与包含反向重复序列的 mdo 启动子区域结合。 mdoR 缺失突变体没有在甲醇上生长，但是通过包含完整的 mdoR 基因的质粒恢复了在甲醇上的生长。在DNase I足迹和EMSA实验中，MdoR与假定的 mdoR 启动子区域中的两个反向重复结合。逆转录PCR显示 mdoR 基因仅在甲醇上生长的细胞中转录，而β-半乳糖苷酶测定表明 mdoR 启动子在甲醇存在下被激活。这些结果表明MdoR充当 mdo 及其自身基因表达的转录激活因子。同样，MdoR是转录调节子TetR家族中第一个被发现的成员，参与了甲醇氧化的调节，并起着积极的自动调节子的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号