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首页> 外文期刊>Journal of bacteriology >FeoB2 Functions in Magnetosome Formation and Oxidative Stress Protection in Magnetospirillum gryphiswaldense Strain MSR-1
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FeoB2 Functions in Magnetosome Formation and Oxidative Stress Protection in Magnetospirillum gryphiswaldense Strain MSR-1

机译：FeoB2在磁螺螺旋藻MSR-1的磁小体形成和氧化应激保护中的作用。

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Magnetotactic bacteria (MTB) synthesize unique organelles, the magnetosomes, which are intracellular nanometer-sized, membrane-enveloped magnetite. The biomineralization of magnetosomes involves the uptake of large amounts of iron. However, the iron metabolism of MTB is not well understood. The genome of the magnetotactic bacterium Magnetospirillum gryphiswaldense strain MSR-1 contains two ferrous iron transport genes, feoB1 and feoB2. The FeoB1 protein was reported to be responsible mainly for the transport of ferrous iron and to play an accessory role in magnetosome formation. To determine the role of feoB2, we constructed an feoB2 deletion mutant (MSR-1 ΔfeoB2) and an feoB1 feoB2 double deletion mutant (MSR-1 NfeoB). The single feoB2 mutation did not affect magnetite crystal biomineralization. MSR-1 NfeoB had a significantly lower average magnetosome number per cell (～65%) than MSR-1 ΔfeoB1, indicating that FeoB2 plays a role in magnetosome formation when the feoB1 gene is deleted. Our findings showed that FeoB1 has a greater ferrous iron transport ability than FeoB2 and revealed the differential roles of FeoB1 and FeoB2 in MSR-1 iron metabolism. Interestingly, compared to the wild type, the feoB mutants showed increased sensitivity to oxidative stress and lower activities of the enzymes superoxide dismutase and catalase, indicating that the FeoB proteins help protect bacterial cells from oxidative stress.
机译：趋磁细菌（MTB）合成独特的细胞器磁小体，它们是细胞内纳米级的膜包裹磁铁矿。磁小体的生物矿化涉及大量铁的吸收。但是，人们对MTB的铁代谢尚不十分了解。趋磁细菌Gryphiswaldense菌株MSR-1的基因组包含两个亚铁转运基因 feoB1 和 feoB2 。据报道，FeoB1蛋白主要负责亚铁的运输，并在磁小体形成中起辅助作用。为了确定 feoB2 的作用，我们构建了一个 feoB2 缺失突变体（MSR-1Δ feoB2 ）和一个 feoB1 feoB2 双缺失突变体（MSR-1 N feoB ）。 feoB2 单一突变不会影响磁铁矿晶体的生物矿化作用。 MSR-1 N feoB 的单细胞平均磁小体数目（〜65％）明显低于MSR-1Δ feoB1 ，表明FeoB2在细胞形成时起着一定的作用 feoB1 基因被删除。我们的发现表明，FeoB1具有比FeoB2更高的亚铁运输能力，并揭示了FeoB1和FeoB2在MSR-1铁代谢中的不同作用。有趣的是，与野生型相比， feoB 突变体对氧化应激表现出更高的敏感性，并且超氧化物歧化酶和过氧化氢酶的活性降低，表明FeoB蛋白有助于保护细菌细胞免受氧化应激的影响。

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