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首页> 外文期刊>Journal of bacteriology >Anaerobic Regulation of Shigella flexneri Virulence: ArcA Regulates fur and Iron Acquisition Genes
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Anaerobic Regulation of Shigella flexneri Virulence: ArcA Regulates fur and Iron Acquisition Genes

机译：弗氏志贺氏菌毒力的厌氧调节：ArcA调节皮毛和铁的获取基因。

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Invasion and plaque formation in epithelial monolayers are routinely used to assess the virulence of Shigella flexneri, a causative agent of dysentery. A modified plaque assay was developed to identify factors contributing to the virulence of S. flexneri under the anaerobic conditions present in the colon. This assay demonstrated the importance of the ferrous iron transport system Feo, as well as the global transcription factors Fur, ArcA, and Fnr, for Shigella plaque formation in anoxic environments. Transcriptional analyses of S. flexneri iron transport genes indicated that anaerobic conditions activated feoABC while repressing genes encoding two other iron transport systems, the ABC transporter Sit and the Iuc/Iut aerobactin siderophore synthesis and transport system. The anaerobic transcription factors ArcA and Fnr activated expression of feoABC, while ArcA repressed iucABCD iutA. Transcription of fur, encoding the iron-responsive transcriptional repressor of bacterial iron acquisition, was also repressed anaerobically in an ArcA-dependent manner.
机译：上皮单层的浸润和斑块形成通常用于评估痢疾的致病菌<志>志贺氏菌的毒力。开发了一种改进的噬斑测定法，以鉴定导致 S毒力的因素。结肠中存在厌氧条件下的弗氏杆菌。该测定法证明了缺氧环境中亚铁转运系统Feo以及全局转录因子Fur，ArcA和Fnr对于Shimella 斑块形成的重要性。对 S的转录分析。 flexneri 铁转运基因表明，厌氧条件激活了 feoABC ，同时抑制了编码其他两个铁转运系统的基因，即ABC转运蛋白Sit和Iuc / Iut航空细菌素铁载体的合成和转运系统。厌氧转录因子ArcA和Fnr激活 feoABC 的表达，而ArcA抑制 iucABCD iutA 。编码细菌铁捕获铁反应性转录阻遏物的 fur 的转录也以ArcA依赖性方式被厌氧抑制。

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