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首页> 外文期刊>Journal of bacteriology >New Functional Identity for the DNA Uptake Sequence in Transformation and Its Presence in Transcriptional Terminators
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New Functional Identity for the DNA Uptake Sequence in Transformation and Its Presence in Transcriptional Terminators

机译：DNA吸收序列在转化中的新功能身份及其在转录终止子中的存在

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The frequently occurring DNA uptake sequence (DUS), recognized as a 10-bp repeat, is required for efficient genetic transformation in the human pathogens Neisseria meningitidis and Neisseria gonorrhoeae. Genome scanning for DUS occurrences in three different species of Neisseria demonstrated that 76% of the nearly 2,000 neisserial DUS were found to have two semiconserved base pairs extending from the 5′ end of DUS to constitute a 12-mer repeat. Plasmids containing sequential variants of the neisserial DUS were tested for their ability to transform N. meningitidis and N. gonorrhoeae, and the 12-mer was found to outperform the 10-mer DUS in transformation efficiency. Assessment of meningococcal uptake of DNA confirmed the enhanced performance of the 12-mer compared to the 10-mer DUS. An inverted repeat DUS was not more efficient in transformation than DNA species containing a single or direct repeat DUS. Genome-wide analysis revealed that half of the nearly 1,500 12-mer DUS are arranged as inverted repeats predicted to be involved in rho-independent transcriptional termination or attenuation. The distribution of the uptake signal sequence required for transformation in the Pasteurellaceae was also biased towards transcriptional terminators, although to a lesser extent. In addition to assessing the intergenic location of DUS, we propose that the 10-mer identity of DUS should be extended and recognized as a 12-mer DUS. The dual role of DUS in transformation and as a structural component on RNA affecting transcription makes this a relevant model system for assessing significant roles of repeat sequences in biology.
机译：为了有效地转化人类病原体脑膜炎奈瑟氏球菌和淋病奈瑟氏球菌，需要将频繁出现的DNA吸收序列（DUS）识别为10 bp的重复序列。基因组扫描发现三种不同的 Neisseria 物种中的DUS发生情况，结果表明，在近2,000种奈瑟氏菌DUS中，有76％具有两个从DUS 5'末端延伸的半保守碱基对，构成一个12-mer重复。测试了含有奈瑟氏菌DUS的顺序变体的质粒转化 N的能力。脑膜炎和 N。淋球菌，发现12聚体的转化效率优于10聚体DUS。评估脑膜炎球菌对DNA的吸收证实，与10聚体DUS相比，12聚体的性能增强。反向重复DUS的转化效率不比包含单个或直接重复DUS的DNA物种高效。全基因组分析表明，将近1,500个12-mer DUS中有一半是反向重复序列，预计与独立于rho的转录终止或衰减有关。在巴氏杆菌科中转化所需的摄取信号序列的分布也偏向转录终止子，尽管程度较小。除了评估DUS的基因间位置外，我们建议应扩展DUS的10-mer身份，并将其识别为12-mer DUS。 DUS在转化中的双重作用以及作为影响转录的RNA的结构成分，使它成为评估重复序列在生物学中的重要作用的相关模型系统。

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